| Survival during cooking and growth from spores of diarrheal and emetic types of Bacillus cereus in rice. | |
| | |
MedLine Citation:
|
PMID: 19903405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bacillus cereus is a gram-positive, spore-forming, facultative anaerobe that is responsible for two types of gastrointestinal diseases: emesis and diarrhea. A significant difference in the D(95 degrees C)-values of spores of the emetic and the diarrheal types was initially determined. A mixture of B. cereus spores of the diarrheal type was inoculated into cooked rice. At inoculation levels of 2.5 x 10(2) spores per g of rice, cell numbers of 6.64 log were detected after 22 h at 20 degrees C and 6.81 log after 34 h at 17 degrees C, whereas at 12 degrees C the counts did not go above 4.0 log even after 48 h. When added to raw rice before cooking at inoculum levels of 10(3)/g, the number of viable spores decreased by 2 log, and a <1-log increase in cell numbers occurred after holding at 20 degrees C for 24 h. In contrast, the emetic spores survived and increased approximately 20-fold. Nonhemolytic enterotoxin was not detected in cooked rice at cell numbers of 8.0 log. Results here provide evidence that the absence of foodborne illness caused by the B. cereus diarrheal biotype with rice as the vehicle is due to the inability of their spores to survive and grow following standard heat processing procedures. |
| | |
Authors:
|
Chandrakant Ankolekar; Ronald G Labb?? |
Related Documents
:
|
10572245 - Heat-induced modulation of lamin b content in two different cell lines. 762195 - The initiation and elongation steps in protein synthesis: relative rates in chinese ham... 6821805 - Evidence for separate modes of action in thermal radiosensitization and direct thermal ... 19670095 - Combination of hyperthermia and bortezomib results in additive killing in mantle cell l... 19805485 - A multiphase model for tissue construct growth in a perfusion bioreactor. 10901435 - Genetic and cellular analysis of male germ cell development. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of food protection Volume: 72 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-11-11 Completed Date: 2010-01-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
|
Languages: eng Pagination: 2386-9 Citation Subset: IM |
Affiliation:
|
Food Microbiology Laboratory, Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus cereus
/
growth & development,
physiology* Colony Count, Microbial Consumer Product Safety Cookery / methods Food Contamination / analysis* Food Handling / methods* Food Microbiology Humans Oryza sativa / microbiology* Spores, Bacterial / growth & development Temperature Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of Temperature and pH on the Growth of Bacteria Isolated from Blown Packs of Vacuum-Packaged...
Next Document: Survival of Hepatitis A Virus in Spinach during Low Temperature Storage.