| Survival and cycle-progression delay of human lymphoma cells in vitro exposed to VP-16-213. | |
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MedLine Citation:
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PMID: 1016966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lethal and kinetic effects of VP-16-213 were analyzed in a human lymphoid cell line (T1 cells) in vitro. When asynchronous T1 cells were exposed to increasing concentrations of VP-16-213 for 1 hour, an exponential survival curve with a Do (mean lethal dose equal to the concentration required to reduce survival by 63% on the exponential part of the survival curve) of 3 mug/ml was obtained. Increasing exposure time also reduced survival exponentially. Synchronized cells showed age-dependent sensitivity to VP-16-213 with the greatest lethal damage experienced by cells treated in S and G2 phase. The major kinetic response of the T1 cells to VP-16-213 was a delay in G2 phase, the extent and duration of which was a function of drug concentration, exposure time, and cell cycle stage of drug addition; thus, cells in S phase were most effectively blocked in the subsequent G2 phase. Continuous treatment with concentrations of VP-16-213 (greater than 0.5 mug/ml for greater than 3 hours) caused a retardation of S-phase transit with prompt recovery after drug release. Treatment with 10.0 mug/ml for greater than 3 hours resulted in a "frozen state" of the whole life cycle, inducing only minor compartment changes. DNA synthesis was inhibited in the majority of cells with an S-phase DNA content. There was no correlation between the extent of perturbation and lethal effects after treatment with VP-16-213. |
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Authors:
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B Drewinko; B Barlogie |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer treatment reports Volume: 60 ISSN: 0361-5960 ISO Abbreviation: Cancer Treat Rep Publication Date: 1976 Sep |
Date Detail:
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Created Date: 1977-04-30 Completed Date: 1977-04-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7607107 Medline TA: Cancer Treat Rep Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1295-1306 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division
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drug effects* Cell Line Cell Survival / drug effects* Dose-Response Relationship, Drug Etoposide / pharmacology* Humans Lymphoma* / pathology Neoplasms, Experimental* / pathology Podophyllotoxin / analogs & derivatives* |
| Chemical | |
Reg. No./Substance:
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33419-42-0/Etoposide; 518-28-5/Podophyllotoxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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