Document Detail


Survival and virulence of Salmonella enterica serovar enteritidis filaments induced by reduced water activity.
MedLine Citation:
PMID:  22287000     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella enterica serovar Enteritidis strain E40 filaments were developed under conditions of a reduced water activity (a(w)) of 0.95 in tryptic soy broth (TSB) or tryptic soy agar (TSA) supplemented with 8% or 7% NaCl, respectively. Filament formation was accompanied by an increase of biomass without an increase in CFU and was affected by incubation temperature and the physical milieu. The greatest amount of filaments was recovered from TSA with 7% NaCl and incubation at 30°C. Within 2 h of transfer to fresh TSB, filaments started to septate into normal-sized cells, resulting in a rapid increase in CFU. S. Enteritidis E40 filaments were not more tolerant of low- or high-temperature stresses than nonfilamented control cells. However, there was greater survival of filaments in 10% bile salts after 24 to 48 h of incubation, during pH 2.0 acid challenge for 10 min, and under desiccation on stainless steel surfaces at 25°C and 75.5% relative humidity for 7 days. S. Enteritidis E40 filaments invaded and multiplied within Caco-2 human intestinal epithelial cells to a similar degree as control cells when a comparable CFU of filaments and control cells was used. S. Enteritidis E40 filaments established a successful infection in mice via intragastric inoculation. The filaments colonized the gastrointestinal tract and disseminated to the spleen and liver at levels comparable to those attained by control cells, even when animals were inoculated with 10- to 100-fold fewer CFU. To our knowledge this is the first demonstration of virulence of stress-induced Salmonella filaments in vitro and in vivo. Formation of filaments by Salmonella in food products and food processing environments is significant to food safety, because detection and quantitation of the pathogen may be compromised. The finding that these filaments are virulent further enhances their potential public health impact.
Authors:
Robert R Stackhouse; Nancy G Faith; Charles W Kaspar; Charles J Czuprynski; Amy C Lee Wong
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-01-27
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-12     Completed Date:  2012-07-17     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2213-20     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, Food Research Institute, University of Wisconsin—Madison, Madison, Wisconsin, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomass
Caco-2 Cells / microbiology
Colony Count, Microbial
Culture Media
Desiccation
Female
Gastrointestinal Tract / microbiology
Heat-Shock Response*
Humans
Hydrogen-Ion Concentration
Mice
Salmonella Infections, Animal / microbiology
Salmonella enteritidis / growth & development*,  pathogenicity*,  ultrastructure
Sodium Chloride / pharmacology
Temperature
Virulence
Water
Chemical
Reg. No./Substance:
0/Culture Media; 7647-14-5/Sodium Chloride; 7732-18-5/Water
Comments/Corrections

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