Document Detail


Survival of Mycobacterium avium subsp. paratuberculosis in synthetic human gastric juice and acidified porcine bile.
MedLine Citation:
PMID:  23263951     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bactericidal activities of synthetic gastric juice and acidified porcine bile on Mycobacterium avium subsp. paratuberculosis were assessed using propidium monoazide (PMA)-mediated quantitative reverse transcription-PCR, which allowed rapid relative quantitative analysis of viable M. avium subsp. paratuberculosis cells.
Authors:
J P Dalton; C Hill
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-21
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  79     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-05     Completed Date:  2013-07-08     Revised Date:  2013-08-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1418-20     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University College Cork, Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Acids / toxicity
Animals
Azides / diagnostic use,  metabolism
Bile / microbiology*
Enzyme Inhibitors / diagnostic use,  metabolism
Gastric Juice / microbiology*
Humans
Microbial Viability*
Models, Theoretical
Mycobacterium avium subsp. paratuberculosis / drug effects,  physiology*
Propidium / analogs & derivatives,  diagnostic use,  metabolism
Real-Time Polymerase Chain Reaction / methods
Swine
Chemical
Reg. No./Substance:
0/Acids; 0/Azides; 0/Enzyme Inhibitors; 0/propidium monoazide; 36015-30-2/Propidium
Comments/Corrections

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