| Survival of hepatitis A virus in spinach during low temperature storage. | |
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MedLine Citation:
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PMID: 19903406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spinach leaves are frequently consumed raw and have been involved with past foodborne outbreaks. In this study, we examined the survival of hepatitis A virus (HAV) on fresh spinach leaves in moisture- and gas-permeable packages that were stored at 5.4 +/- 1.2 degrees C for up to 42 days. Different eluents including phosphate-buffered saline (PBS), pH 7.5 (with and without 2% serum), and 3% beef extract (pH 7.5 and 8) were compared for how efficiently they recovered viruses from spinach by using a simple elution procedure (<1 h). The recoveries were compared and determined by a plaque assay with FRhK-4 cells. Culture grade PBS containing 2% serum was found to be appropriate for HAV elution from spinach leaves, with an average recovery of 45% +/- 10%. Over 4 weeks of storage at 5.4 +/- 1.2 degrees C, HAV in spinach decreased slightly more than 1 log, with 6.75% of the original titer remaining. HAV survived under refrigerated temperatures on spinach leaves with a D-value of 28.6 days (equivalent to an inactivation rate of -0.035 log of HAV per day, r(2) = 0.88). In comparison, HAV in PBS containing 2% serum under the same storage conditions remained constant throughout 7 weeks. The inactivation rate of -0.035 log each day for HAV on spinach leaves was possibly due to the interaction of the virus and the leaf. |
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Authors:
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Y Carol Shieh; Diana S Stewart; David T Laird |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of food protection Volume: 72 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-11 Completed Date: 2010-01-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 2390-3 Citation Subset: IM |
Affiliation:
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U.S. Food and Drug Administration, National Center for Food Safety and Technology, Summit-Argo, Illinois 60501, USA. carol.shieh@fda.hhs.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cold Temperature Consumer Product Safety* Culture Media / chemistry Food Contamination / analysis* Food Microbiology Food Preservation / methods* Hepatitis A virus / growth & development*, isolation & purification Humans Hydrogen-Ion Concentration Plant Leaves / virology Public Health Spinacia oleracea / virology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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