Document Detail


Survival of Bacillus cereus Vegetative Cells and Spores during In Vitro Simulation of Gastric Passage.
MedLine Citation:
PMID:  22488056     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The enteric pathogen Bacillus cereus must survive gastric passage in order to cause diarrhea by enterotoxin production in the small intestine. The acid resistance and the survival after gastric passage were assessed by in vitro experiments with acidified growth medium and gastric simulation medium with B. cereus NVH 1230-88 vegetative cells and spores. First, batch incubations at constant pH values for 4 h, which represented different physiological states of the stomach, showed that spores were resistant to any gastric condition in the pH range of 2.0 to 5.0, while vegetative cells were rapidly inactivated at pH values of ≤4.0. Second, a dynamic in vitro gastric experiment was conducted that simulated the continuously changing in vivo conditions due to digestion dynamics by gradually decreasing the pH from 5.0 to 2.0 and fractional emptying of the stomach 30 to 180 min from the start of the experiment. All of the B. cereus spores and 14% (± 9%) of the vegetative cells survived the dynamic simulation of gastric passage.
Authors:
Siele Ceuppens; Mieke Uyttendaele; Katrien Drieskens; Andreja Rajkovic; Nico Boon; Tom Van de Wiele
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  75     ISSN:  1944-9097     ISO Abbreviation:  J. Food Prot.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  690-4     Citation Subset:  IM    
Affiliation:
Faculty of Bioscience Engineering, Department of Food Safety and Quality, Laboratory of Food Microbiology and Food Preservation (LFMFP), Ghent University, Ghent, Belgium; Faculty of Bioscience Engineering, Department of Biochemical and Microbial Technology, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Ghent, Belgium.
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