Document Detail


Surveillance methodology for Vibrio cholerae in environmental samples.
MedLine Citation:
PMID:  16854675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to examine the prevalence of Vibrio cholerae in environmental water samples by using a series of biochemical tests. A total of 223 V. cholerae-like bacteria were isolated from TCBS agar after spreading the alkaline peptone water enriched sewer (n = 21) and water (n = 16) samples. All oxidase positive isolates were subjected to confirmation for V. cholerae by seven other biochemical tests and polymerase chain reaction. Only 74.2% isolates were found to be V. cholerae by PCR using primers against an outer membrane protein (ompW) gene, out of which only 2 isolates were positive for cholera toxin (ctxAB) gene. Among the various biochemical tests studied, arginine hydrolysis, arabinose fermentation and string test showed 92 - 100% sensitivity and 42 - 67% specificity. Eight isolates including the toxigenic ones, showed agglutination with V. cholerae O1 antiserum. The present study showed that no biochemical test is 100% specific for V. cholerae. However, a few tests, if performed in a sequence after growing the alkaline peptone water enriched samples onto TCBS media can be used for screening of V. cholerae from the environmental samples. This study also showed that most of the environmental isolates are non-O1/non-O139 and the chances of presence of toxigenic V. cholerae are very rare in the environment.
Authors:
Akhilesh K Tamrakar; Ajay K Goel; Dev V Kamboj; Lokendra Singh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of environmental health research     Volume:  16     ISSN:  0960-3123     ISO Abbreviation:  Int J Environ Health Res     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-20     Completed Date:  2006-10-03     Revised Date:  2009-08-14    
Medline Journal Info:
Nlm Unique ID:  9106628     Medline TA:  Int J Environ Health Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  305-12     Citation Subset:  IM    
Affiliation:
Biotechnology Division, Defence Research & Development Establishment, Gwalior, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Outer Membrane Proteins / genetics
Base Sequence
Cholera / epidemiology*,  microbiology,  transmission
Cholera Toxin / genetics,  metabolism,  toxicity
Environmental Monitoring*
Humans
Polymerase Chain Reaction
Population Surveillance / methods*
Vibrio cholerae / classification,  genetics,  isolation & purification*
Vibrio cholerae O1 / genetics,  isolation & purification
Vibrio cholerae O139 / genetics,  isolation & purification
Water Microbiology*
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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