Document Detail


Surveillance of dairy production holdings supplying raw milk to the farmhouse cheese sector for Escherichia coli O157, O26 and O111.
MedLine Citation:
PMID:  18035974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clinically healthy domestic animals can harbour Escherichia coli O157 and other verocytotoxigenic E. coli (VTEC) strains in their faeces. Milk filters can be used to microbiologically monitor direct milk secretion and environmental contamination for these pathogens. The aim of this study was to establish baseline data on the prevalence and characteristics of VTEC organisms in lactating animals (bovine, ovine and caprine) supplying milk to the farmhouse cheese sector, with particular emphasis on serogroups O157, O111 and O26. Fifty-six bovine, 13 caprine and 5 ovine herds/flocks, the majority of which supplying milk for farmhouse cheese production, were surveyed from May 2004 to July 2005. Milk filters were analysed by immunomagnetic separation followed by PCR, on a serogroup-specific basis for E. coli O157, O26 and O111. Positive isolates were examined using a multiplex PCR protocol, for their potential to produce verocytotoxins (vt1/vt2), the haemolysin-encoding gene (hlyA) and the gene encoding attaching and effacement (eae). Five verocytotoxigenic and 22 non-virulent E. coli O157 isolates were detected. Seventeen E. coli O26 isolates were also detected, four of which were verocytotoxigenic, seven isolates contained the eae gene only and six isolates were devoid of any of the virulence factors. The VTEC O157 and O26 isolates contained the hlyA and eae genes along with the verocytotoxin genes. No E. coli O111 isolates were detected. Some of the herds were positive on more than one occasion and multiple E. coli serogroups were isolated from the same milk filter sample. Although all food products tested were VTEC negative, routine surveillance for such pathogens in raw milk/raw milk products is of public health importance. Herd-level surveillance along with subsequent risk management action may be a cost-effective component of risk reduction strategies for food production, drinking water supplies and the protection of public health.
Authors:
M Murphy; J F Buckley; P Whyte; M O'Mahony; W Anderson; P G Wall; S Fanning
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zoonoses and public health     Volume:  54     ISSN:  1863-1959     ISO Abbreviation:  Zoonoses Public Health     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-26     Completed Date:  2008-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101300786     Medline TA:  Zoonoses Public Health     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  358-65     Citation Subset:  IM    
Affiliation:
Veterinary Food Safety Laboratory, Cork County Council, Inniscarra, Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cattle Diseases / epidemiology,  microbiology,  transmission
Cheese / microbiology
Dairying / methods
Escherichia coli* / classification,  genetics,  isolation & purification
Escherichia coli Infections / epidemiology,  microbiology,  transmission,  veterinary*
Escherichia coli O157 / classification,  genetics,  isolation & purification
Female
Goat Diseases / epidemiology,  microbiology,  transmission
Goats
Humans
Mastitis / epidemiology,  microbiology,  veterinary*
Mastitis, Bovine / epidemiology,  microbiology,  transmission
Milk / microbiology*
Phylogeny
Population Surveillance
Prevalence
Public Health
Serotyping
Sheep
Sheep Diseases / epidemiology,  microbiology,  transmission
Shiga Toxins / biosynthesis
Zoonoses
Chemical
Reg. No./Substance:
0/Shiga Toxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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