| Surgical ventricular restoration with a cell- and cytokine-seeded biodegradable scaffold. | |
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MedLine Citation:
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PMID: 20659765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Late after a myocardial infarction (MI), surgical ventricular restoration (SVR) can reduce left ventricular volumes, but an enhanced cardiac patch may be required to restore function. We developed a new, biodegradable patch (modified gelfoam, MGF) consisting of a spongy inner core (gelfoam) to encourage cell engraftment and an outer coating (poly epsilon-caprolactone) to provide sufficient strength to permit ventricular repair. Two weeks after coronary ligation in rats, SVR was performed using one of the following: gelfoam, MGF, MGF patches with hydrogel alone, or with hydrogel and cytokines (stem cell factor, stromal cell-derived factor-1alpha), bone marrow mesenchymal stem cells, or both. Cardiac function and morphology were evaluated by echocardiography, conduction catheterization, magnetic resonance imaging, and histology. Animals whose hearts were repaired with untreated gelfoam died of ventricular rupture. The MGF groups had significantly improved myocardial systolic function vs. MI controls. Enhancement with cytokines and/or cells promoted more alpha-smooth muscle actin-positive cells, more capillaries, greater wall thickness, a more ellipsoid shape, greater fractional shortening, and better-preserved systolic elastance than MGF alone. This combination of the new, reinforced, biodegradable biomaterial and cytokine/cell treatment created a viable tissue after SVR and produced better functional outcomes than un-reinforced gelfoam or MGF alone. |
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Authors:
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Yasuo Miyagi; Faquan Zeng; Xi-Ping Huang; Warren D Foltz; Jun Wu; Anton Mihic; Terrence M Yau; Richard D Weisel; Ren-Ke Li |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-24 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-20 Completed Date: 2011-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 7684-94 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Division of Cardiovascular Surgery, Toronto General Hospital, University Health Network and the University of Toronto, Toronto, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / chemistry, metabolism Bone Marrow Cells / cytology, physiology Cytokines / chemistry, metabolism* Female Gelatin Sponge, Absorbable / chemistry, metabolism Heart Ventricles* / pathology, surgery Humans Hydrogels / chemistry, metabolism Materials Testing Molecular Structure Myocardial Infarction* / pathology, surgery Polyesters / chemistry, metabolism Rats Rats, Sprague-Dawley Stromal Cells / cytology, physiology Tissue Engineering* / instrumentation, methods Tissue Scaffolds / chemistry* Ventricular Function, Left |
| Grant Support | |
ID/Acronym/Agency:
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RMF82498//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Cytokines; 0/Hydrogels; 0/Polyesters; 24980-41-4/polycaprolactone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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