| Surgical protocol involving the infusion of paramagnetic microparticles for preferential incorporation within porcine islets. | |
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MedLine Citation:
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PMID: 21168666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Despite significant advances, widespread applicability of islet cell transplantation remains elusive. Refinement of current islet isolation protocols may improve transplant outcomes. Islet purification by magnetic separation has shown early promise. However, surgical protocols must be optimized to maximize the incorporation of paramagnetic microparticles (MP) within a greater number of islets. This study explores the impact of MP concentration and infusion method on optimizing MP incorporation within islets. METHODS: Five porcine pancreata were procured from donors after cardiac death. Splenic lobes were isolated and infused with varying concentrations of MP (8, 16, and 32 × 10(8) MP/L of cold preservation solution) and using one of two delivery techniques (hanging bag versus hand-syringe). After procurement and infusion, pancreata were stored at 0°C to 4°C during transportation (less than 1 hour), fixed in 10% buffered formalin, and examined by standard magnetic resonance imaging (MRI) and histopathology. RESULTS: T2*-weighted MRI showed homogeneous distribution of MP in all experimental splenic lobes. In addition, histologic analysis confirmed that MP were primarily located within the microvasculature of islets (82% to 85%), with few MP present in acinar tissue (15% to 18%), with an average of five to seven MP per islet (within a 5-μm thick section). The highest MP incorporation was achieved at a concentration of 16 × 10(8) MP/L using the hand-syringe technique. CONCLUSION: This preliminary study suggests that optimization of a surgical protocol, MP concentrations, and applied infusion pressures may enable more uniform distribution of MP in the porcine pancreas and better control of MP incorporation within islets. These results may have implications in maximizing the efficacy of islet purification by magnetic separation. |
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Authors:
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M D Rizzari; T M Suszynski; L S Kidder; S A Stein; T D O'Brien; V S K Sajja; W E Scott; V A Kirchner; B P Weegman; E S Avgoustiniatos; P W Todd; D J Kennedy; B E Hammer; D E R Sutherland; B J Hering; K K Papas |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation proceedings Volume: 42 ISSN: 1873-2623 ISO Abbreviation: Transplant. Proc. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-20 Completed Date: 2011-04-25 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 4209-12 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota 55455, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Islets of Langerhans / pathology Islets of Langerhans Transplantation / methods* Magnetic Resonance Imaging Microspheres* Swine |
| Grant Support | |
ID/Acronym/Agency:
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2R44DK072647-02A1/DK/NIDDK NIH HHS; R01 DK068717-02/DK/NIDDK NIH HHS; R44 DK072647-02A1/DK/NIDDK NIH HHS; R44 DK072647-03/DK/NIDDK NIH HHS |
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