Document Detail


Surfactin: a novel mosquitocidal biosurfactant produced by Bacillus subtilis ssp. subtilis (VCRC B471) and influence of abiotic factors on its pupicidal efficacy.
MedLine Citation:
PMID:  20796211     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The rpoB gene of the mosquito pupicidal isolate Bacillus subtilis (VCRC B471) was amplified to confirm the subspecies as subtilis. The mosquito pupicidal activity expressed by the biosurfactant surfactin is novel, and hence, the influence of abiotic factors like pH, temperature of water and sunlight on its efficacy was studied under laboratory conditions.
METHODS AND RESULTS: The rpoB gene amplicon of the bacterium (c. 570 bp of) was sequenced (accession number: EU057603). The relatedness of the bacterium to other members of the genus Bacillus was studied by tree construction, and the identity of VCRC B471 was confirmed as B. subtilis ssp. subtilis. The mosquito pupicidal activity exhibited by surfactin was found to be unaffected between pH 3-9, temperatures 25 and 37 °C and exposure to sunlight/UV radiation. Further, the pupicidal activity of surfactin was not diminished after exposure to 121 °C for 15 min, indicating its thermostable nature.
CONCLUSIONS: VCRC B471 is confirmed as a strain of B. subtilis ssp. subtilis. The mosquitocidal toxin, surfactin produced by this bacterium being stable to UV and varied temperature, active at acidic and basic pH and temperatures between 25 and 42 °C renders this molecule an interesting lead to be developed as a mosquitocidal agent.
SIGNIFICANCE AND IMPACT OF THE STUDY: The mosquitocidal toxin, surfactin produced by B. subtilis ssp. subtilis (VCRC B471), being a biodegradable biosurfactant, exhibiting high stability to varied environmental conditions, can be used year round in breeding habitats and will be a prospective microbial toxin for use against mosquitoes.
Authors:
I Geetha; A M Manonmani
Publication Detail:
Type:  Journal Article     Date:  2010-08-26
Journal Detail:
Title:  Letters in applied microbiology     Volume:  51     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2011-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  406-12     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology.
Affiliation:
Unit of Microbiology and Immunology, Vector Control Research Centre, Indian Council of Medical Research (ICMR), Indira Nagar, Puducherry, India. iglory@yahoo.com
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU057603
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MeSH Terms
Descriptor/Qualifier:
Animals
Anopheles / drug effects*,  growth & development*
Bacillus subtilis / classification,  genetics,  metabolism*
Biological Assay
Hydrogen-Ion Concentration
Insecticides / chemistry,  isolation & purification,  metabolism*,  pharmacology*
Molecular Sequence Data
Pupa / drug effects,  growth & development
Sequence Analysis, DNA
Sunlight
Surface-Active Agents / chemistry,  isolation & purification,  metabolism*,  pharmacology*
Temperature
Water / chemistry
Chemical
Reg. No./Substance:
0/Insecticides; 0/Surface-Active Agents; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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