Document Detail

Surfactin isoforms from Bacillus subtilis HSO121: separation and characterization.
MedLine Citation:
PMID:  18336355     Owner:  NLM     Status:  MEDLINE    
Natural surfactin is a mixture of cyclic lipopeptides built from variants of a heptapeptide and a beta-hydroxy fatty acid. A biosurfactant-producing strain, Bacillus subtilis HSO121, was isolated from the production water of an oil field. The strain was able to produce eight surfactin isoforms which have been isolated by acid-precipitation followed by extraction into methanol. A novel procedure for the purification of surfactin was achieved. It consists of a solid-phase extraction on C(18) gel followed by reversed-phase high performance liquid chromatography using Prep. HiQ sil C18W, column. The surfactin isoforms were eluted by linear acetonitrile gradient from 80-100%. The peaks were analyzed by TLC on silica gel, and after acid hydrolysis their amino acid compositions were determined by HPLC analysis. Eight isoforms of surfactin had nearly the same amino acid composition and appeared a single spot in TLC. According to the R(f) values with the amino acid composition, these peaks belong to the surfactin group of lipopeptides. Infrared analysis of the purified samples also revealed a pattern similar to that of surfactin. This is a very effective method for isolating and fractionating lipopeptides, of the same or different nature.
Namir I A Haddad; Xiangyang Liu; Shizhong Yang; Bozhong Mu
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protein and peptide letters     Volume:  15     ISSN:  0929-8665     ISO Abbreviation:  Protein Pept. Lett.     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-13     Completed Date:  2008-07-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9441434     Medline TA:  Protein Pept Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  265-9     Citation Subset:  IM    
State Key laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China.
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MeSH Terms
Bacillus subtilis / metabolism*
Chromatography, High Pressure Liquid
Peptides, Cyclic / chemistry*,  isolation & purification*
Reg. No./Substance:
0/Lipopeptides; 0/Peptides, Cyclic; 24730-31-2/surfactin peptide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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