Document Detail

Surfactant to dye binding degree based approach for the selective determination of L-glutamate in foodstuffs.
MedLine Citation:
PMID:  17952418     Owner:  NLM     Status:  MEDLINE    
A selective method for the determination of L-glutamate in foodstuffs has been developed. It was based on the competition established between the analyte and the dye Coomassie brilliant blue G (CBBG) to interact with the surfactant didodecyldimethylammonium bromide (DDABr). The measurement parameter was the amount of DDABr required to reach a given dye-to-surfactant binding degree. It was obtained by photometric titration on the basis of the changes observed in the spectral characteristics of the dye when CBBG-DDABr aggregates were formed. The calibration graph obtained was linear in the L-glutamate concentration interval 0.2-5 mM (detection limit 0.05 mM). The high selectivity of the proposed method (other amino acids and food additives did not interfere at the concentrations present in foodstuffs) permitted the direct analysis of food samples after dissolution of the analyte in hot water. The accuracy of the surfactant to the dye binding degree method was demonstrated by determining L-glutamate in different kinds of foodstuffs (liquid and dried soups, seasonings, pasta sauces and dried mushroom creams) and comparing the results obtained with those provided by the commercial Boehringer Mannheim essay.
Ana Pedraza; María Dolores Sicilia; Soledad Rubio; Dolores Pérez-Bendito
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-19
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  389     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-30     Completed Date:  2008-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2297-302     Citation Subset:  IM    
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Edificio Anexo Marie Curie, Campus de Rabanales, 14071, Córdoba, Spain.
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MeSH Terms
Food Analysis / methods*,  standards
Glutamic Acid / analysis*,  chemistry
Indicators and Reagents
Quality Control
Quaternary Ammonium Compounds / chemistry,  metabolism*
Rosaniline Dyes / chemistry,  metabolism*
Surface-Active Agents* / chemistry,  metabolism
Reg. No./Substance:
0/Indicators and Reagents; 0/Quaternary Ammonium Compounds; 0/Rosaniline Dyes; 0/Surface-Active Agents; 13146-86-6/didodecyldimethylammonium; 56-86-0/Glutamic Acid; 6104-58-1/coomassie Brilliant Blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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