Document Detail


Surfactant proteins B and C are both necessary for alveolar stability at end expiration in premature rabbits with respiratory distress syndrome.
MedLine Citation:
PMID:  18276900     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Modified natural surfactant preparations, used for treatment of respiratory distress syndrome in premature infants, contain phospholipids and the hydrophobic surfactant protein (SP)-B and SP-C. Herein, the individual and combined effects of SP-B and SP-C were evaluated in premature rabbit fetuses treated with airway instillation of surfactant and ventilated without positive end-expiratory pressure. Artificial surfactant preparations composed of synthetic phospholipids mixed with either 2% (wt/wt) of porcine SP-B, SP-C, or a synthetic poly-Leu analog of SP-C (SP-C33) did not stabilize the alveoli at the end of expiration, as measured by low lung gas volumes of approximately 5 ml/kg after 30 min of ventilation. However, treatment with phospholipids containing both SP-B and SP-C/SP-C33 approximately doubled lung gas volumes. Doubling the SP-C33 content did not affect lung gas volumes. The tidal volumes were similar in all groups receiving surfactant. This shows that SP-B and SP-C exert different physiological effects, since both proteins are needed to establish alveolar stability at end expiration in this animal model of respiratory distress syndrome, and that an optimal synthetic surfactant probably requires the presence of mimics of both SP-B and SP-C.
Authors:
Andreas Almlén; Guido Stichtenoth; Bim Linderholm; Marie Haegerstrand-Björkman; Bengt Robertson; Jan Johansson; Tore Curstedt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-14
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  104     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-03     Completed Date:  2008-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1101-8     Citation Subset:  IM    
Affiliation:
Dept. of Molecular Medicine and Surgery, Section of Clinical Chemistry, Bldg. L2:03, Karolinska Univ. Hospital, SE-171 76 Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
1,2-Dipalmitoylphosphatidylcholine / pharmacology
Amino Acid Sequence
Animals
Animals, Newborn / physiology*
Female
Humans
Infant, Newborn
Lung Volume Measurements
Molecular Sequence Data
Phospholipids / metabolism
Positive-Pressure Respiration
Pregnancy
Pulmonary Alveoli / physiology*
Pulmonary Surfactant-Associated Protein B / physiology*
Pulmonary Surfactant-Associated Protein C / physiology*
Pulmonary Surfactants / pharmacology
Rabbits
Respiratory Distress Syndrome, Newborn / physiopathology*
Respiratory Mechanics / physiology
Tidal Volume / physiology
Chemical
Reg. No./Substance:
0/Phospholipids; 0/Pulmonary Surfactant-Associated Protein B; 0/Pulmonary Surfactant-Associated Protein C; 0/Pulmonary Surfactants; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine

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