Document Detail

Surfactant protein A in bronchoalveolar lavage fluid.
MedLine Citation:
PMID:  1500824     Owner:  NLM     Status:  MEDLINE    
We measured surfactant protein A and phosphatidylcholine in the bronchoalveolar lavage fluid from healthy volunteers and several groups of patients with lung diseases to obtain information on surfactant in the lung. We developed three types of enzyme-linked immunosorbent assays that used combinations of polyclonal antiserum and monoclonal antibodies. Phosphatidylcholine was assessed by enzymatic measurement. The median amounts of surfactant protein A in bronchoalveolar lavage fluid determined by the enzyme-linked immunosorbent assay with monoclonal antibodies were as follows: control subjects (n = 10), 2.82 mg/L (range, 0.92 to 5.17 mg/L); patients with asthma (n = 13), 1.89 mg/L (range, 0.45 to 2.95 mg/L); and patients with pulmonary sarcoidosis (n = 20), 2.98 mg/L (range, 0.68 to 7.02 mg/L). The median phosphatidylcholine concentrations in the bronchoalveolar lavage fluid were as follows: control subjects (n = 10), 20 mumol/L (range, 3 to 37 mumol/L); patients with asthma (n = 12), 24 mumol/L (range, 3 to 55 mumol/L); and patients with pulmonary sarcoidosis (n = 20), 26 mumol/L (range, 4 to 76 mumol/L). As a group, the patients with asthma had less surfactant protein A in bronchoalveolar lavage fluid than did the control subjects (Mann-Whitney U test, p less than 0.05). The surfactant protein A levels measured by the enzyme-linked immunosorbent assay with polyclonal antiserum and by a competitive enzyme-linked immunosorbent assay were also lower in bronchoalveolar lavage fluid from patients with asthma than in that from control subjects. The phosphatidylcholine concentrations in all groups were similar.(ABSTRACT TRUNCATED AT 250 WORDS)
E A van de Graaf; H M Jansen; R Lutter; C Alberts; J Kobesen; I J de Vries; T A Out
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  120     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-09-11     Completed Date:  1992-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  252-63     Citation Subset:  AIM; IM    
Department of Pulmonology, Academic Medical Center, Amsterdam, Netherlands.
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MeSH Terms
Antibodies, Monoclonal
Asthma / physiopathology*
Blotting, Western
Bronchoalveolar Lavage Fluid / chemistry*
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Lung Diseases / physiopathology*
Molecular Weight
Phosphatidylcholines / analysis*
Proteolipids / analysis*
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactants / analysis*
Reference Values
Sarcoidosis / physiopathology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Phosphatidylcholines; 0/Proteolipids; 0/Pulmonary Surfactant-Associated Proteins; 0/Pulmonary Surfactants

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