Document Detail

Surfactant protein-A-deficient mice are susceptible to Pseudomonas aeruginosa infection.
MedLine Citation:
PMID:  9761768     Owner:  NLM     Status:  MEDLINE    
To determine the role of surfactant protein-A (SP-A) in host defense, the murine SP-A locus was targeted by homologous recombination to produce mice lacking SP-A. SP-A-/- and wild-type mice were infected with mucoid Pseudomonas aeruginosa by intratracheal instillation. Pulmonary bacterial loads were greater in SP-A-/- than in wild-type mice, with increased numbers of mucoid P. aeruginosa in lung homogenates at 6 and 24 h after infection. Pulmonary infiltration with polymorphonuclear leukocytes (PMN) was similar in both groups; however, an earlier influx of PMN into the lung occurred in the SP-A-/- mice. The number of bacteria phagocytosed by alveolar macrophages was decreased in the SP-A-/- mice at 1 h after infection. Superoxide-radical generation by PMN was similar for the SP-A-/- and wild-type mice, but nitrite levels were increased in SP-A-/- mice. Concentrations of tumor necrosis factor-alpha, interleukin-6, and macrophage inflammatory protein-2 (proinflammatory cytokines) were greater in bronchoalveolar lavage fluid at 2 h after infection in SP-A-/- mice. SP-A plays an important role in the pathogenesis of mucoid P. aeruginosa infection in the lung in vivo by enhancing macrophage phagocytosis and clearance of bacteria, and by modifying the inflammatory response.
A M LeVine; K E Kurak; M D Bruno; J M Stark; J A Whitsett; T R Korfhagen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  19     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-11-05     Completed Date:  1998-11-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  700-8     Citation Subset:  IM    
Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio, USA.
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MeSH Terms
Bronchoalveolar Lavage Fluid / immunology,  microbiology
Chemokine CXCL2
Chemotactic Factors / metabolism
Glycoproteins / genetics
Interleukin-1 / metabolism
Interleukin-6 / metabolism
Macrophages, Alveolar / immunology,  metabolism,  microbiology
Mice, Knockout
Monokines / metabolism
Neutrophils / immunology,  metabolism,  microbiology
Nitrites / metabolism
Phagocytosis / immunology
Pneumonia, Bacterial / immunology*,  microbiology*,  pathology
Proteolipids / genetics*
Pseudomonas Infections / immunology*,  pathology
Pseudomonas aeruginosa*
Pulmonary Surfactant-Associated Protein A
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactants / genetics*
Superoxides / metabolism
Tumor Necrosis Factor-alpha / metabolism
Grant Support
Reg. No./Substance:
0/Chemokine CXCL2; 0/Chemotactic Factors; 0/Glycoproteins; 0/Interleukin-1; 0/Interleukin-6; 0/Monokines; 0/Nitrites; 0/Proteolipids; 0/Pulmonary Surfactant-Associated Protein A; 0/Pulmonary Surfactant-Associated Proteins; 0/Pulmonary Surfactants; 0/Tumor Necrosis Factor-alpha; 11062-77-4/Superoxides

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