| Surfactant protein D modulates pulmonary clearance of pollen starch granules. | |
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MedLine Citation:
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PMID: 20815657 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pollen starch granules (PSGs) are allergen particles that get into contact with pulmonary surfactant and phagocytes in the terminal airways. In this study, the effects of surfactant protein D (SP-D) on the interaction of PSGs with phagocytes and on the pulmonary clearance of PSGs were determined. Fluorescently labeled PSGs were incubated in vitro with murine lung macrophages or dendritic cells (DCs) ± recombinant rat SP-D (rrSP-D). In addition, the effect of SP-D on uptake of PSGs by lung macrophages and DCs was studied in vivo. Furthermore, PSGs were instilled in Balb/c mice and the effects of SP-D on total lung clearance were assessed by optical imaging. SP-D treatment increased the number of PSG-positive macrophages and DCs in vitro. Furthermore, SP-D accelerated uptake/binding by alveolar macrophages and reduced the number of PSG-positive tissue macrophages and DCs at 24 hours. However, SP-D did not affect total lung clearance of PSGs and it did not enhance the T-cell proliferation induced by PSG-positive DCs. In conclusion, SP-D increased PSG-positive cells in vitro and accelerated PSG binding/uptake in vivo. The observed effects were limited to cellular clearance mechanisms and did not affect the total clearance of PSGs from the lung. |
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Authors:
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Carla Winkler; Katja Hüper; Ann-Carina Wedekind; Sabine Rochlitzer; Christina Hartwig; Meike Müller; Armin Braun; Norbert Krug; Jens M Hohlfeld; Veit J Erpenbeck |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental lung research Volume: 36 ISSN: 1521-0499 ISO Abbreviation: Exp. Lung Res. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-13 Completed Date: 2011-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8004944 Medline TA: Exp Lung Res Country: England |
Other Details:
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Languages: eng Pagination: 522-30 Citation Subset: IM |
Affiliation:
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Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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immunology,
metabolism* Animals Cell Proliferation / drug effects Cells, Cultured Female Flow Cytometry Lung / drug effects*, immunology, metabolism Mice Mice, Inbred BALB C Microscopy, Fluorescence Mucociliary Clearance / drug effects* Phagocytosis / drug effects, immunology Pollen / immunology, metabolism* Pulmonary Surfactant-Associated Protein D / pharmacology* Rats Recombinant Proteins Starch / immunology, metabolism* T-Lymphocytes / cytology, drug effects |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Pulmonary Surfactant-Associated Protein D; 0/Recombinant Proteins; 9005-25-8/Starch |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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