| Surfactant phosphatidylcholine half-life and pool size measurements in premature baboons developing bronchopulmonary dysplasia. | |
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MedLine Citation:
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PMID: 12409520 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Because minimal information is available about surfactant metabolism in bronchopulmonary dysplasia, we measured half-lives and pool sizes of surfactant phosphatidylcholine in very preterm baboons recovering from respiratory distress syndrome and developing bronchopulmonary dysplasia, using stable isotopes, radioactive isotopes, and direct pool size measurements. Eight ventilated premature baboons received (2)H-DPPC (dipalmitoyl phosphatidylcholine) on d 5 of life, and radioactive (14)C-DPPC with a treatment dose of surfactant on d 8. After 14 d, lung pool sizes of saturated phosphatidylcholine were measured. Half-life of (2)H-DPPC (d 5) in tracheal aspirates was 28 +/- 4 h (mean +/- SEM). Half-life of radioactive DPPC (d 8) was 35 +/- 4 h. Saturated phosphatidylcholine pool size measured with stable isotopes on d 5 was 129 +/- 14 micro mol/kg, and 123 +/- 11 micro mol/kg on d 14 at autopsy. Half-lives were comparable to those obtained at d 0 and d 6 in our previous baboon studies. We conclude that surfactant metabolism does not change during the early development of bronchopulmonary dysplasia, more specifically, the metabolism of exogenous surfactant on d 8 is similar to that on the day of birth. Surfactant pool size is low at birth, increases after surfactant therapy, and is kept constant during the first 2 wk of life by endogenous surfactant synthesis. Measurements with stable isotopes are comparable to measurements with radioactive tracers and measurements at autopsy. |
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Authors:
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Daphne J M T Janssen; Virgilio P Carnielli; Paola E Cogo; Steven R Seidner; Ingrid H I Luijendijk; J L Darcos Wattimena; Alan H Jobe; Luc J I Zimmermann |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Pediatric research Volume: 52 ISSN: 0031-3998 ISO Abbreviation: Pediatr. Res. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-31 Completed Date: 2003-03-31 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
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Languages: eng Pagination: 724-9 Citation Subset: IM |
Affiliation:
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Department of Pediatrics/Neonatology, Sophia's Children's Hospital/Erasmus University Rotterdam, Rotterdam, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1,2-Dipalmitoylphosphatidylcholine
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pharmacokinetics* Animals Animals, Newborn Bronchoalveolar Lavage Fluid / chemistry Bronchopulmonary Dysplasia / metabolism* Carbon Radioisotopes / pharmacokinetics Convalescence Deuterium / pharmacokinetics Female Half-Life Humans Infant, Newborn Lung / metabolism* Male Papio Pulmonary Surfactants / metabolism*, therapeutic use Respiratory Distress Syndrome, Newborn / metabolism, therapy Trachea |
| Grant Support | |
ID/Acronym/Agency:
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HL 52635/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Pulmonary Surfactants; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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