Document Detail

Surfactant-enhanced desorption/ionization on silicon mass spectrometry.
MedLine Citation:
PMID:  16383337     Owner:  NLM     Status:  MEDLINE    
Perfluorinated surfactants are demonstrated to dramatically enhance desorption/ionization on fluorinated silicon (DIOS) mass spectrometry. Perfluorooctanesulfonic acid improved the signal-to-noise ratio of tryptic digests and gave a 3-fold increase in the number of peptides identified. Similar results were also obtained using perfluoroundecanoic acid; yet among the seven different surfactants tested, controls such as nonfluorinated sodium dodecyl sulfate or fluorinated molecules with minimal surfactant activity did not enhance the signal. The same surfactants also enhanced the DIOS-MS signal of amino acids, carbohydrates, and other small organic compounds. The signal enhancement may be facilitated by the high surface activity of the perfluorinated surfactants on the fluorinated silicon surfaces allowing for a higher concentration of analyte to be absorbed.
Anders Nordström; Junefredo V Apon; Wilasinee Uritboonthai; Eden P Go; Gary Siuzdak
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  78     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-30     Completed Date:  2007-04-12     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  272-8     Citation Subset:  IM    
Department of Molecular Biology, The Scripps Center for Mass Spectrometry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
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MeSH Terms
Alkanesulfonic Acids / chemistry
Fluorocarbons / chemistry
Peptide Fragments / analysis*
Pulmonary Surfactants / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Surface-Active Agents / pharmacology*
Trypsin / pharmacology
Grant Support
Reg. No./Substance:
0/Alkanesulfonic Acids; 0/Fluorocarbons; 0/Peptide Fragments; 0/Pulmonary Surfactants; 0/Surface-Active Agents; 1763-23-1/perfluorooctane sulfonic acid; 7440-21-3/Silicon; EC

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