Document Detail


Surfactant protein-A as an anti-inflammatory component in the amnion: implications for human pregnancy.
MedLine Citation:
PMID:  20439915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanism of mouse parturition is thought to involve myometrial infiltration by amniotic fluid (AF) macrophages, activated by surfactant protein-A (SP-A). In humans, the concentration of AF SP-A decreases during labor, and no fetal macrophages are found in the myometrium after labor. Therefore, it appears that the mechanisms of labor in mice and humans are different. We investigated a potential role for SP-A in human pregnancy and parturition by examining SP-A expression patterns in AF and amnion. High molecular mass (>250 kDa) oligomeric SP-A was increased in AF with advancing gestation. Interestingly, these oligomers were more abundant in placental amnion before labor at term, while they increased primarily in reflected amnion during labor (p < 0.05). Immunoblotting showed a binding of high molecular mass SP-A in AF to amnion. In C57BL/6 mice, oligomeric SP-A was also readily detected in AF from E15 onwards, but not in amnion. Macrophage density in mice myometrium did not change with advancing gestational age. Microarray analysis of human amnion explants incubated with SP-A revealed a molecular signature of inhibited cytokine-cytokine receptor interaction with downregulation of IL-1beta, CXCL2, and CXCL5 mRNA expression. The findings in this study strongly suggest that SP-A signals amniotic anti-inflammatory response via AF during pregnancy. We propose that an SP-A interaction among AF, placental amnion, and reflected amnion is a unique mechanism for immunoregulation in human pregnancy akin to that established in lung biology. However, AF SP-A and fetal macrophages by themselves do not seem to be exclusive effectors of parturition in humans.
Authors:
Deug-Chan Lee; Roberto Romero; Chong Jai Kim; Tinnakorn Chaiworapongsa; Adi L Tarca; JoonHo Lee; Yeon-Lim Suh; Shali Mazaki-Tovi; Edi Vaisbuch; Pooja Mittal; Sorin Draghici; Offer Erez; Juan Pedro Kusanovic; Sonia S Hassan; Jung-Sun Kim
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2010-05-03
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  184     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-20     Completed Date:  2010-06-14     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6479-91     Citation Subset:  AIM; IM    
Affiliation:
Perinatology Research Branch, Department of Health and Human Services, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, USA.
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MeSH Terms
Descriptor/Qualifier:
Amnion / immunology*
Amniotic Fluid / immunology*
Animals
Blotting, Western
Cell Separation
Chromatography, Liquid
Female
Flow Cytometry
Fluorescent Antibody Technique
Humans
Immunoprecipitation
Inflammation Mediators / immunology*,  metabolism
Macrophages / immunology
Mass Spectrometry
Mice
Mice, Inbred C57BL
Myometrium / immunology
Oligonucleotide Array Sequence Analysis
Parturition / immunology*
Pregnancy / immunology*
Pulmonary Surfactant-Associated Protein A / analysis,  immunology*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
ZIA HD002400-19/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Inflammation Mediators; 0/Pulmonary Surfactant-Associated Protein A
Comments/Corrections

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