Document Detail


Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity.
MedLine Citation:
PMID:  20810986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During pulmonary infections, a careful balance between activation of protective host defense mechanisms and potentially injurious inflammatory processes must be maintained. Surfactant protein A (SP-A) is an immune modulator that increases pathogen uptake and clearance by phagocytes while minimizing lung inflammation by limiting dendritic cell (DC) and T cell activation. Recent publications have shown that SP-A binds to and is bacteriostatic for Mycoplasma pneumoniae in vitro. In vivo, SP-A aids in maintenance of airway homeostasis during M. pneumoniae pulmonary infection by preventing an overzealous proinflammatory response mediated by TNF-α. Although SP-A was shown to inhibit maturation of DCs in vitro, the consequence of DC/SP-A interactions in vivo has not been elucidated. In this article, we show that the absence of SP-A during M. pneumoniae infection leads to increased numbers of mature DCs in the lung and draining lymph nodes during the acute phase of infection and, consequently, increased numbers of activated T and B cells during the course of infection. The findings that glycyrrhizin, a specific inhibitor of extracellular high-mobility group box-1 (HMGB-1) abrogated this effect and that SP-A inhibits HMGB-1 release from immune cells suggest that SP-A inhibits M. pneumoniae-induced DC maturation by regulating HMGB-1 cytokine activity.
Authors:
Julie G Ledford; Bernice Lo; Michele M Kislan; Joseph M Thomas; Katherine Evans; Derek W Cain; Monica Kraft; Kristi L Williams; Jo Rae Wright
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-09-01
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  185     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2010-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3884-94     Citation Subset:  AIM; IM    
Affiliation:
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Differentiation / immunology
Cell Separation
Dendritic Cells / cytology,  immunology*,  metabolism
Flow Cytometry
HMGB1 Protein / immunology*,  metabolism
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Mycoplasma Infections / immunology*,  metabolism
Mycoplasma pneumoniae / immunology
Pulmonary Surfactant-Associated Protein A / immunology*,  metabolism
Grant Support
ID/Acronym/Agency:
AI81672/AI/NIAID NIH HHS; F32HL091642/HL/NHLBI NIH HHS; HL084917/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/HMGB1 Protein; 0/Pulmonary Surfactant-Associated Protein A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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