Document Detail


Surfactant protein D-mediated decrease of allergen-induced inflammation is dependent upon CTLA4.
MedLine Citation:
PMID:  20435925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pulmonary surfactant protein D (SP-D), a member of the collectin family, is an innate immune molecule critical for defense that can also modulate adaptive immune responses. We previously showed that SP-D-deficient mice exhibit enhanced allergic responses and that SP-D induction requires lymphocytes. Thus, we postulated that SP-D may decrease adaptive allergic responses through interaction with T cells. In this study, we used two forms of SP-D, a dodecamer and a shorter fragment containing the trimeric neck and carbohydrate recognition domains (SP-D NCRD). Both forms decreased immune responses in vitro and in a murine model of pulmonary inflammation. SP-D NCRD increased transcription of CTLA4, a negative regulator of T cell activation, in T cells. SP-D NCRD no longer decreased lymphoproliferation and IL-2 cytokine production when CTLA4 signals were abrogated. Administration of SP-D NCRD in vivo no longer decreased allergen induced responses when CTLA4 was inhibited. Our results indicate that SP-D decreases allergen responses, an effect that may be mediated by increase of CTLA4 in T cells.
Authors:
Ko-Wei Lin; Kai Yu Jen; Carlos Jose Suarez; Erika C Crouch; David L Perkins; Patricia W Finn
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-04-30
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  184     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-20     Completed Date:  2010-06-14     Revised Date:  2012-02-01    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6343-9     Citation Subset:  AIM; IM    
Affiliation:
Division of Pulmonary and Critical Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology
Animals
Antigens, CD / immunology*
CTLA-4 Antigen
Enzyme-Linked Immunosorbent Assay
Immunoglobulin E / blood,  immunology
Inflammation / immunology*
Lymphocyte Activation / immunology
Mice
Mice, Inbred BALB C
Ovalbumin / immunology
Pulmonary Surfactant-Associated Protein D / immunology*
Respiratory Hypersensitivity / immunology*
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes / immunology*
Transfection
Grant Support
ID/Acronym/Agency:
5R01HL081663-05/HL/NHLBI NIH HHS; R01 AI053878-08/AI/NIAID NIH HHS; R01 AI053878-10/AI/NIAID NIH HHS; R01 AI075317-02/AI/NIAID NIH HHS; R01 HL081663-01/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, CD; 0/CTLA-4 Antigen; 0/Ctla4 protein, mouse; 0/Pulmonary Surfactant-Associated Protein D; 37341-29-0/Immunoglobulin E; 9006-59-1/Ovalbumin
Comments/Corrections

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