Document Detail


Surfactant protein-d inhibits lung inflammation caused by ventilation in premature newborn lambs.
MedLine Citation:
PMID:  20133924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: Premature newborns frequently require manual ventilation for resuscitation during which lung injury occurs. Although surfactant protein (SP)-D regulates pulmonary inflammation, SP-D levels are low in the preterm lung. Commercial surfactants for treatment of respiratory distress syndrome do not contain SP-D.
OBJECTIVES: To determine whether addition of recombinant human SP-D (rhSP-D) to commercial surfactant influences lung inflammation in ventilated premature newborn lambs.
METHODS: Prematurely delivered lambs (130 d gestation age) were resuscitated with 100% O(2) and peak inspiratory pressure 40 cm H(2)O for 20 minutes and then treated with Survanta or Survanta containing rhSP-D. Ventilation was then changed to regulate tidal volume at 8 to 9 ml/kg. At 5 hours of age lambs were killed for sample collection.
MEASUREMENTS AND MAIN RESULTS: Sequential blood gas and tidal volume were similar in lambs treated with or without rhSP-D, indicating that lung immaturity and ventilatory stress used to support premature lambs were comparable between the two groups. Ventilation caused pulmonary inflammation in lambs treated with surfactant alone. In contrast, surfactant containing rhSP-D decreased neutrophil numbers in bronchoalveolar lavage fluid and decreased neutrophil elastase activity in lung tissue. IL-8 mRNA and IL-8 protein were significantly decreased in the +rhSP-D group lamb lungs, to 20% of those in controls. The addition of rhSP-D also rendered Survanta more resistant to plasma protein inhibition of surfactant function.
CONCLUSIONS: Treatment with rhSP-D-containing surfactant inhibited lung inflammation and enhanced the resistance of surfactant to inhibition, supporting its potential usefulness for prevention of lung injury in the preterm newborn.
Authors:
Atsuyasu Sato; Jeffrey A Whitsett; Ronald K Scheule; Machiko Ikegami
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-02-04
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  181     ISSN:  1535-4970     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-06-09     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1098-105     Citation Subset:  AIM; IM    
Affiliation:
Cincinnati Children's Hospital Medical Center, Division of Pulmonary Biology, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Female
Humans
Lung / physiopathology
Male
Pneumonia / etiology,  physiopathology,  prevention & control*
Pulmonary Surfactant-Associated Protein D / pharmacology*
Recombinant Proteins / pharmacology
Respiration, Artificial / adverse effects
Sheep
Ventilator-Induced Lung Injury / prevention & control*
Grant Support
ID/Acronym/Agency:
HL085610/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Pulmonary Surfactant-Associated Protein D; 0/Recombinant Proteins
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