Document Detail

Surface ultrastructure of intact and in situ chlorhexidine-treated human buccal cells. A method for scanning electron microscopy.
MedLine Citation:
PMID:  9370024     Owner:  NLM     Status:  MEDLINE    
Air-dried and ethanol-fixed buccal epithelial cell smears from five subjects were observed by scanning electron microscopy. The mucous pellicle was precipitated as a smooth haze covering the cells, and outlines of bacteria were found embedded within it. Rinsing the preparations under running water gradually diminished the mucous pellicle but not the cell-adherent bacteria. A more complete dissolution of the pellicle was accomplished by washing the buccal epithelial cells before smearing. After a chlorhexidine mouthrinse the buccal cells appeared distorted, with only a few adherent bacteria. Three days after the rinsing, the denatured appearance still persisted on many cells, however, simultaneously with the emergence of undenatured epithelial cells with adherent bacteria. The method introduced in this study is useful to investigate the bacteria-mucus-epithelial cell interactions. A possible mode of antibacterial activity of chlorhexidine in vivo may be that it destroys bacterial adhesins. The substantivity of chlorhexidine in the oral cavity may be linked to the turnover rate of the oral epithelial cells.
L H Vaahtoniemi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta odontologica Scandinavica     Volume:  55     ISSN:  0001-6357     ISO Abbreviation:  Acta Odontol. Scand.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-12-23     Completed Date:  1997-12-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370344     Medline TA:  Acta Odontol Scand     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  277-81     Citation Subset:  D; IM    
Department of Oral and Maxillofacial Surgery, University of Oulu, Finland.
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MeSH Terms
Adhesins, Bacterial / drug effects
Anti-Infective Agents, Local / pharmacology*
Bacteria / drug effects,  ultrastructure
Bacterial Adhesion
Cells, Cultured
Chemical Precipitation
Chlorhexidine / pharmacology*
Epithelial Cells / drug effects,  ultrastructure
Microscopy, Electron, Scanning
Mouth Mucosa / cytology,  drug effects,  ultrastructure*
Mouthwashes / pharmacology*
Mucus / drug effects
Salivary Proteins and Peptides / drug effects,  ultrastructure
Time Factors
Tissue Fixation
Reg. No./Substance:
0/Adhesins, Bacterial; 0/Anti-Infective Agents, Local; 0/Fixatives; 0/Mouthwashes; 0/Salivary Proteins and Peptides; 55-56-1/Chlorhexidine; 64-17-5/Ethanol

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