Document Detail

Surface properties of bacteria from activated sludge in relation to bioflocculation.
MedLine Citation:
PMID:  21462699     Owner:  NLM     Status:  In-Process    
Two bacterial stains were isolated from the activated sludge and identified as Leucobacter sp. and Alcaligenesfaecalis by 16S rDNA sequencing. Pure cultures of these two strains, representing well or poorly settled bacteria, were used to investigate the mechanism of bioflocculation in activated sludge. Based on the analyses of the characteristics of cells hydrophobicity, zeta-potential, flocculation ability and extracellular polymeric substance (EPS) composition under different growth stages, it was found that the ratio of cell EPS protein had the highly influence on zeta-potential and hydrophobicity, which were important factors to bioflocculation. Cellulase and Proteinase K could destroy the extracellular biopolymer and resulted in a decrease in the hydrophobicity and zeta-potential. However, in our study, the flocculation characteristics exhibited differently in relation to cellulase and Proteinase K. Flocculation of cells treated with cellulase and Proteinase K decreased sharply, and then recovered quickly in cellulase treatment, while cells treated with Proteinase K showed no sign of recovery. This reveals that the presence of protein in extracellular biopolymer plays an important role to the bioflocculation of cells.
Bing Xie; Jidong Gu; Jun Lu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of environmental sciences (China)     Volume:  22     ISSN:  1001-0742     ISO Abbreviation:  J Environ Sci (China)     Publication Date:  2010  
Date Detail:
Created Date:  2011-04-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100967627     Medline TA:  J Environ Sci (China)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1840-5     Citation Subset:  IM    
Key Laboratory of Urbanization and Ecological Restoration of Shanghai, Department of Environmental Science & Technology, East China Normal University, Shanghai 200062, China.
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