| Surface plasmon resonance measurement of pH-induced responses of immobilized biomolecules: conformational change or electrostatic interaction effects? | |
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MedLine Citation:
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PMID: 12381366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, the observation of pH-induced conformational changes of biomolecules supported on carboxymethyldextran (CMD)-coated surfaces measured using surface plasmon resonance (SPR) has been reported. However, it is apparent that the evidence reported in the literature is ambiguous. The research presented in this paper describes investigations to study the changing SPR signal of immobilized biomolecules as a function of varying pH, to provide a detailed understanding of the origin of the pH-induced changes in the SPR profile. SPR measurements were performed with cytochrome c, concanavalin A, and poly-L-lysine, biomolecules that exhibit diverse conformational responses to changing pH, covalently immobilized onto CMD-coated supports. These SPR measurements were supported by circular dichroism (CD) solution studies. The SPR profiles recorded were not consistent with the conformational transitions of the biomolecules as observed using CD. An alternative explanation for the observed shifts in SPR is proposed, which explains the SPR profiles in terms of electrostatic interaction effects between the immobilized biomolecules and the carboxymethyldextran matrix. |
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Authors:
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Sally Paynter; David A Russell |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 309 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-10-16 Completed Date: 2003-05-01 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 85-95 Citation Subset: IM |
Affiliation:
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School of Chemical Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Canavalia / chemistry Circular Dichroism Concanavalin A / chemistry*, classification Cytochrome c Group / chemistry* Dextrans / chemistry Horses Hydrogen-Ion Concentration Myocardium / chemistry Peptides Polylysine / chemistry* Protein Conformation Protein Folding Reference Standards Static Electricity Surface Plasmon Resonance / methods* |
| Chemical | |
Reg. No./Substance:
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0/Cytochrome c Group; 0/Peptides; 11028-71-0/Concanavalin A; 25104-18-1/Polylysine; 9004-54-0/Dextrans; 9044-05-7/carboxymethyl dextran |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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