Document Detail


Surface mucopolysaccharides of Treponema pallidum.
MedLine Citation:
PMID:  156696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The viscous mucoid fluid that accumulates within syphilitic lesions may be due to breakdown of host tissue during infection, or may be synthesized by Treponema pallidum. Experiments were performed to investigate the acidic mucopolysaccharides that occur at the surface of T. pallidum (Nichols strain). These mucopolysaccharides were demonstrated by reaction with acidified bovine serum albumin and by agglutination with wheat germ agglutinin and soybean agglutinin. The polycations ruthenium red and toluidine blue influenced treponemal survival. Concentrations of both compounds at 200 mug/ml inhibited survival, whereas concentrations at 0.1mug/ml enhanced survival. The mucopolysaccharide concentration within the mucoid fluid that accumulates during intratesticular infection was determined by reaction with acidified bovine serum albumin; it ranged from 10,000 mug/ml to less than 8 mug/ml. The addition of this mucoid fluid to treponemal suspensions resulted in differing effects on T. pallidum survival. Some preparations were inhibitory, and others were stimulatory. Commercial preparations of hyaluronic acid and chondroitin sulfate at 400, 200, 100, and 50 mug/ml were detrimental to treponemal survival. The organisms exhibited pronounced clumping in the presence of the higher concentrations of hyaluronic acid. These clumps of treponemes were comprised of mucopolysaccharides as shown by acidified bovine serum albumin and toluidine blue reactions and by hyaluronidase degradation. Results are discussed in terms of the derivation and potential role of acidic mucopolysaccharides at the surface of T. pallidum.
Authors:
T J Fitzgerald; R C Johnson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  24     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1979 Apr 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  244-51     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Extracellular Space / microbiology
Glycosaminoglycans / analysis*
Isoelectric Point
Lectins
Male
Movement
Rabbits
Syphilis / microbiology*
Testis / microbiology
Treponema pallidum / analysis*
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Lectins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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