| Surface modification of titanium-based alloys with bioactive molecules using electrochemically fixed nucleic acids. | |
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MedLine Citation:
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PMID: 16680695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method of surface modification for titanium (alloys) with bioactive molecules was developed with the intention of providing a new basis of implant adaptation for particular requirements of certain medical indications. Nucleic acid single strands are fixed electrochemically via their termini (regiospecifically) by growing an oxide layer on Ti6Al7Nb anodically. It could be shown that they are accessible to subsequent hybridization with complementary strands at physiological pH. Amount of nucleic acids immobilized and hybridized were determined radioanalytically using 32P-labelled nucleic acids. Stable fixation was attained at and above potentials of 4 V(SCE). Up to 4 pmol/cm2 of nucleic acid single strands could be immobilized and hybridization efficiencies up to 1.0 were reached. Hybridization efficiency was found to depend on surface density of immobilized oligonucleotides, while hybridization rates increased when MgCl2 was added. A conjugate consisting of an oligonucleotide complementary to the immobilized strand and the hexapeptide GRGDSP with RGD as an integrin recognition site was synthesized. This conjugate was able to bind to integrins on osteoblasts. It was shown that this conjugate binds to the anchor strand fixed on Ti6Al7Nb to an extent comparable with the unconjugated complementary strand. |
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Authors:
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J Michael; R Beutner; U Hempel; D Scharnweber; H Worch; B Schwenzer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part B, Applied biomaterials Volume: 80 ISSN: 1552-4973 ISO Abbreviation: J. Biomed. Mater. Res. Part B Appl. Biomater. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-12 Completed Date: 2007-02-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234238 Medline TA: J Biomed Mater Res B Appl Biomater Country: United States |
Other Details:
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Languages: eng Pagination: 146-55 Citation Subset: IM |
Copyright Information:
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2006 Wiley Periodicals, Inc. |
Affiliation:
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Institut für Biochemie, Technische Universität Dresden, Bergstr. 66, 01069 Dresden, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Cell Culture Techniques Cells, Cultured Coated Materials, Biocompatible / chemistry* Electrochemistry Integrins / chemistry Materials Testing Nucleic Acid Hybridization Oligonucleotides / chemistry* Oligopeptides / chemistry* Osteoblasts / cytology* Rats Surface Properties Titanium / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Coated Materials, Biocompatible; 0/Integrins; 0/Oligonucleotides; 0/Oligopeptides; 105657-12-3/Ti-6Al-7Nb alloy; 7440-32-6/Titanium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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