Document Detail


Surface modification of biodegradable polyesters with fatty acid conjugates for improved drug targeting.
MedLine Citation:
PMID:  15878378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a general method for incorporating target ligands into the surface of biocompatible polyester poly(lactic-co-glycolic acid) (PLGA) 50/50 materials using fatty acids. Avidin-fatty acid conjugates were prepared and efficiently incorporated into PLGA. Avidin was chosen as an adaptor protein to facilitate the attachment of a variety of biotinylated ligands. We show that fatty acid preferentially associates with the hydrophobic PLGA matrix, rather than the external aqueous environment, facilitating a prolonged presentation of avidin over several weeks. We successfully applied this approach in both microspheres encapsulating a model protein, bovine serum albumin, and PLGA scaffolds fabricated by a salt-leaching method. Because of its ease, generality and flexibility, this strategy promises widespread utility in modifying the surface of PLGA-based materials for applications in drug delivery and tissue engineering.
Authors:
Tarek M Fahmy; Robert M Samstein; Casey C Harness; W Mark Saltzman
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-04-18
Journal Detail:
Title:  Biomaterials     Volume:  26     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-05-09     Completed Date:  2005-10-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  5727-36     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Yale University, PO Box 208284, New Haven, CT 06520, USA. Tarek.fahmy@yale.edu
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MeSH Terms
Descriptor/Qualifier:
Absorbable Implants*
Avidin / administration & dosage,  analysis,  chemistry*
Coated Materials, Biocompatible / analysis,  chemistry*
Diffusion
Drug Delivery Systems / methods*
Fatty Acids / chemistry*
Lactic Acid / analysis,  chemistry*
Materials Testing
Microspheres
Particle Size
Polyesters / chemistry
Polyglycolic Acid / analysis,  chemistry*
Polymers / analysis,  chemistry*
Surface Properties
Grant Support
ID/Acronym/Agency:
EB00487/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Fatty Acids; 0/Polyesters; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 1405-69-2/Avidin; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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