Document Detail

Surface hydrolysis of polyacrylonitrile with nitrile hydrolysing enzymes from Micrococcus luteus BST20.
MedLine Citation:
PMID:  17223216     Owner:  NLM     Status:  MEDLINE    
A new Micrococcus luteus strain BST20 was isolated with ability to metabolize PAN polymers as sole carbon source. Out of seven synthetized PAN copolymers containing different moieties of acrylic acid and/or vinyl acetate the polymer with lowest crystallinity (PAN with 5% vinyl acetate) was most easily metabolized. (13)C labelled PAN was completely converted to the acrylic acid by this strain. M. luteus BST20 produced membrane-bound nitrile hydrolysing enzymes able to convert nitrile groups on PAN powder surface to the corresponding acids. Similarly, nitrile groups on PAN fabrics were transformed to the corresponding acid as indicated by an K/S increased after dying with Methylene blue and the released ammonia. On small soluble substrates the enzyme system showed a preference for aliphatic and aromatic substituted aliphatic nitriles.
Gudrun Fischer-Colbrie; Teresa Matama; Sonja Heumann; Ludmila Martinkova; Artur Cavaco Paulo; Georg Guebitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-02
Journal Detail:
Title:  Journal of biotechnology     Volume:  129     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-19     Completed Date:  2007-05-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  62-8     Citation Subset:  IM    
Graz University of Technology, Department of Environmental Biotechnology, Petersgasse 12/I A, 8010 Graz, Austria.
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MeSH Terms
Acrylic Resins / metabolism*
Magnetic Resonance Spectroscopy
Micrococcus luteus / enzymology*,  growth & development
Nitriles / metabolism*
Polymers / metabolism
Substrate Specificity
Surface Properties
Reg. No./Substance:
0/Acrylic Resins; 0/Nitriles; 0/Polymers; 25014-41-9/polyacrylonitrile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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