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Surface functions during mitosis. II. Quantitation of pinocytosis and kinetic characterization of the mitotic cycle with a new fluorescence technique.
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MedLine Citation:
PMID:  6156175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The profound depression of fluid pinocytosis observed in mitotic cells (Berlin, R. D., et al. 1978. Cell. 15:327--341) is documented by quantitative microspectrofluorimetry of fluorescein-labeled dextran uptake in single cells. In J774.2 macrophages, fluid pinocytosis is reduced 30-fold during mitosis. The depression develops within 30 s of entry into prophase and recovers with equal rapidity upon emergence from telophase into G1. This characteristic pattern of fluid pinocytosis forms the basis of a new method for detailed kinetic analysis of the duration of mitosis and its phases. The analysis is applied to the J774.2 macrophage cell line but should be generally applicable to other lines. Effects of ouabain and colchicine on the length of mitosis and its phases are evaluated, revealing a selective prolongation of metaphase by ouabain and suggesting a role for microtubules in the transition from G2 into mitosis.
Authors:
R D Berlin; J M Oliver
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  85     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1980 Jun 
Date Detail:
Created Date:  1980-09-26     Completed Date:  1980-09-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  660-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / drug effects
Cell Line
Colchicine / pharmacology
Dextrans
Interphase / drug effects
Macrophages / physiology*,  ultrastructure
Mice
Mitosis* / drug effects
Ouabain / pharmacology
Pinocytosis* / drug effects
Time Factors
Chemical
Reg. No./Substance:
630-60-4/Ouabain; 64-86-8/Colchicine; 9004-54-0/Dextrans
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 6 Year: 1980
Volume: 85 Issue: 3
First Page: 660 Last Page: 671
ID: 2111441
Publisher Id: 80228028
PubMed Id: 6156175

Surface functions during mitosis. II. Quantitation of pinocytosis and kinetic characterization of the mitotic cycle with a new fluorescence technique


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