| Surface functionalization of superparamagnetic nanoparticles for the development of highly efficient magnetic resonance probe for macrophages. | |
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MedLine Citation:
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PMID: 21287679 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In vivo magnetic resonance imaging (MRI) tracking of macrophages plays an important role in monitoring and understanding numerous human diseases with high macrophage activity. In this work, superparamagnetic iron oxide nanoparticles (SPIONs) of ∼12 nm were surface-functionalized with poly(DL-lactic acid-co-malic acid) copolymer (PLMA) via a nanoprecipitation method. The r(1) , r(2) and r(2) /r(1) values of the PLMA-SPIONs obtained at a magnetic field of 3 T were 0.38, 196 and 516 mM(-1) s(-1) , respectively. The high r(2) /r(1) ratio can be expected to provide enhanced MR contrast. The PLMA-SPIONs were readily taken in by macrophages and the high iron uptake was confirmed via Prussian Blue staining and quantified by inductively coupled plasma mass spectrometry (ICP-MS). No significant cytotoxicity was found even at a high nanoparticle loading of 67.7 pg Fe per cell. A linear relationship between R(2) and R 2* values and the number of PLMA-SPIONs labeled cells was observed in vitro. As a result of the significantly higher R 2* than R(2) effects, an in vitro detection threshold of about 2820 labeled cells was achieved with short labeling time and low nanoparticle concentration using a clinical 3 T MRI scanner. Thus, the PLMA-SPIONs can be potentially useful as magnetic resonance probes for targeting and tracking macrophages. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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Chao Huang; Koon Gee Neoh; Liang Wang; En-Tang Kang; Borys Shuter |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-2 |
Journal Detail:
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Title: Contrast media & molecular imaging Volume: - ISSN: 1555-4317 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101286760 Medline TA: Contrast Media Mol Imaging Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 119260. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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