Document Detail


Surface functionalization regulates PAMAM dendrimer toxicity on Blood Brain Barrier cells and the modulation of key inflammatory receptors on microglia.
MedLine Citation:
PMID:  23298388     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Dendrimers are branched polymers with spherical morphology. Their tunable chemistry and surface modification make them valuable nanomaterials for biomedical applications. In view of possible dendrimer uses as brain-aimed nanocarriers we studied amine- and lipid-functionalized (G4) polyamidoamine (PAMAM) biocompatibility with cell population forming the Blood Brain Barrier (BBB). Both PAMAM dendrimers were able to enter endothelial and primary neural cells. However, only amine- PAMAM damaged cell membranes in a dose dependent manner. Transmission electron microscopy (TEM) evidenced the ability of dendrimers to precipitate salts and serum components present in culture medium that slightly increased toxicity of the amine-PAMAM. Amine- and lipid-PAMAM were both able to cross the BBB and differently induced CD11b and CCR2 over-expression on primary CX3CR1-GFP murine microglia in vitro. These data emphasize the role of dendrimer surface functionalization in toxicity and neural immune cell activation, raising concerns about possible neuroinflammatory reactions.
Authors:
Alice Bertero; Adriano Boni; Mauro Gemmi; Mariacristina Gagliardi; Angelo Bifone; Giuseppe Bardi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  Nanotoxicology     Volume:  -     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Istituto Italiano di Tecnologia, Center for Nanotechnology Innovation, Piazza San Silvestro 12, Pisa, Italy, 56127.
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