Document Detail

Surface functionalization affects the zeta potential, coronal stability and membranolytic activity of polymeric nanoparticles.
MedLine Citation:
PMID:  23379633     Owner:  NLM     Status:  Publisher    
Abstract Nano materials are commonly functionalized to boost their physicochemical properties. However, there is little known about the impact of these modifications on cellular systems. Herein, we synthesized 8 types of polymeric nanoparticles bearing different functional groups, and investigated their effects on interactions with cellular membranes. As models for particle- membrane interactions, hemolysis assays using human red blood cells and culture with A549 cells were utilized. Under protein-free conditions, the nanoparticles showed a wide distribution of zeta potentials which showed a good correlation with their hemolytic potential. However, in the presence of serum or lung lining fluid, the zeta potentials of all nanoparticles coalesced towards a single common negative value and showed neither hemolytic activity nor cytotoxicity to A549 cells. Lipase and protease treatment of the coronated particles did not restore their reactivity. These results imply that particle functionalization influences the stability of the particle corona which, if intact, prevents hemolytic activity and membrane disrupture.
Frank Thielbeer; Ken Donaldson; Mark Bradley; Emma M V Johansson; Wan-Seob Cho; Rodger Duffin; Ian L Megson; William Macnee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-5
Journal Detail:
Title:  Nanotoxicology     Volume:  -     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
School of Chemistry, Science and Engineering, 21, Joseph Black Building, West Mains Road, Edinburgh, United Kingdom, EH9 3JJ.
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