Document Detail


Surface expression and rapid internalization of macrosialin (mouse CD68) on elicited mouse peritoneal macrophages.
MedLine Citation:
PMID:  10648004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Macrosialin, the mouse homolog of human CD68, is a heavily glycosylated transmembrane protein found almost exclusively in macrophages. Its function remains uncertain. It has a high affinity for oxidized low-density lipoprotein (LDL) in ligand blots and antibodies against the human homolog, CD68, inhibit the binding of oxidized LDL to a human monocyte-derived cell line (THP-1). However, there is still controversy as to whether macrosialin, found predominantly in late endosomes, is expressed at all on the plasma membrane. The present studies, done in thioglycollate-elicited peritoneal macrophages, confirm that macrosialin is predominantly intracellular but show clearly that 10-15% of it is expressed on the cell surface. Exchange with intracellular pools occurs at an extremely high rate. The results are compatible with a surface function, including internalization of bound ligands or adhesion to surfaces.
Authors:
H Kurushima; M Ramprasad; N Kondratenko; D M Foster; O Quehenberger; D Steinberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  67     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-09     Completed Date:  2000-02-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  104-8     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of California, San Diego, La Jolla 92093-0682, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / metabolism*
Antigens, Differentiation, Myelomonocytic / metabolism*
Cells, Cultured
Humans
Lipoproteins, LDL / metabolism
Macrophages, Peritoneal / metabolism*
Mice
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; 0/CD68 protein, mouse; 0/Lipoproteins, LDL; 0/oxidized low density lipoprotein

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