| Surface enhanced bacterial fluorescence and enumeration of bacterial adhesion. | |
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MedLine Citation:
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PMID: 23185995 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The use of flow displacement systems for studying initial bacterial adhesion to surfaces is mostly confined to transparent substrata. The objective of this study was to investigate a method based on macroscopic fluorescence imaging to enumerate adhering fluorescent bacteria on non-transparent substrata, real-time and under flow. To this end, a stepwise protocol is described to quantify adhesion of green-fluorescent-protein producing Staphylococcus aureus on polished and non-polished metal and polymer surfaces accounting for surface-enhanced-fluorescence on metal surfaces, quantified by the ratio of the single cell fluorescence observed for adhering and planktonic bacteria. Enumeration of adhering fluorescent staphylococci by the proposed method is consistent with results obtained using metallurgical microscopy. An advantage however, is that the non-homogeneous surface coverage and surface roughness do not limit the applicability of the method. Moreover, the accurate quantification of surface-enhanced-fluorescence arising from adhering bacteria offers a new pathway for evaluating bacterial cell surface deformation during adhesion. |
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Authors:
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Jiuyi Li; Henk J Busscher; Henny C van der Mei; Jelmer Sjollema |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biofouling Volume: 29 ISSN: 1029-2454 ISO Abbreviation: Biofouling Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2012-11-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9200331 Medline TA: Biofouling Country: England |
Other Details:
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Languages: eng Pagination: 11-9 Citation Subset: IM |
Affiliation:
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a Department of Biomedical Engineering , W.J. Kolff Institute, University Medical Center and University of Groningen , Antonius Deusinglaan 1, 9713 AV Groningen , The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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