| Surface display of acid protease on the cells of Yarrowia lipolytica for milk clotting. | |
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MedLine Citation:
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PMID: 20349181 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The acid protease structural gene was amplified from the genomic DNA of Saccharomycopsis fibuligera A11. When the gene was cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in the cells of Yarrowia lipolytica, the cells displaying the acid protease could form clear zone on the plate-containing milk indicating that they had extracellular acid protease activity. The cells displaying the acid protease can be used to effectively clot skimmed milk. The highest clotting milk activity (1,142.9 U/ml) was observed under the conditions of pH 3.0, 40 degrees C, 20 mM of CaCl(2), and 10% skimmed milk powder. We found that the acid protease displayed on the cells of Y. lipolytica which has generally regarded as safe status could be easily isolated and concentrated compared to the free acid protease. Therefore, the displayed acid protease may have many potential applications in food and cheese industries. This is the first report that the yeast cells displaying the acid protease were used to clot milk. |
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Authors:
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Xin-Jun Yu; Catherine Madzak; Hui-Juan Li; Zhen-Ming Chi; Jing Li |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-27 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 87 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-24 Completed Date: 2010-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 669-77 Citation Subset: IM |
Affiliation:
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UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartic Acid Endopeptidases / genetics, metabolism* Cattle Cell Membrane / genetics, metabolism* Fungal Proteins / genetics, metabolism* Gene Expression* Milk / chemistry*, microbiology Protein Transport Saccharomycopsis / enzymology*, genetics Yarrowia / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; EC 3.4.23.-/Aspartic Acid Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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