Document Detail


Surface display of acid protease on the cells of Yarrowia lipolytica for milk clotting.
MedLine Citation:
PMID:  20349181     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The acid protease structural gene was amplified from the genomic DNA of Saccharomycopsis fibuligera A11. When the gene was cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in the cells of Yarrowia lipolytica, the cells displaying the acid protease could form clear zone on the plate-containing milk indicating that they had extracellular acid protease activity. The cells displaying the acid protease can be used to effectively clot skimmed milk. The highest clotting milk activity (1,142.9 U/ml) was observed under the conditions of pH 3.0, 40 degrees C, 20 mM of CaCl(2), and 10% skimmed milk powder. We found that the acid protease displayed on the cells of Y. lipolytica which has generally regarded as safe status could be easily isolated and concentrated compared to the free acid protease. Therefore, the displayed acid protease may have many potential applications in food and cheese industries. This is the first report that the yeast cells displaying the acid protease were used to clot milk.
Authors:
Xin-Jun Yu; Catherine Madzak; Hui-Juan Li; Zhen-Ming Chi; Jing Li
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-27
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  87     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-09-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  669-77     Citation Subset:  IM    
Affiliation:
UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartic Acid Endopeptidases / genetics,  metabolism*
Cattle
Cell Membrane / genetics,  metabolism*
Fungal Proteins / genetics,  metabolism*
Gene Expression*
Milk / chemistry*,  microbiology
Protein Transport
Saccharomycopsis / enzymology*,  genetics
Yarrowia / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Fungal Proteins; EC 3.4.23.-/Aspartic Acid Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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