Document Detail


Surface binding of aflatoxin B(1) by lactic acid bacteria.
MedLine Citation:
PMID:  11425726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Specific lactic acid bacterial strains remove toxins from liquid media by physical binding. The stability of the aflatoxin B(1) complexes formed with 12 bacterial strains in both viable and nonviable (heat- or acid-treated) forms was assessed by repetitive aqueous extraction. By the fifth extraction, up to 71% of the total aflatoxin B(1) remained bound. Nonviable bacteria retained the highest amount of aflatoxin B(1). Lactobacillus rhamnosus strain GG (ATCC 53103) and L. rhamnosus strain LC-705 (DSM 7061) removed aflatoxin B(1) from solution most efficiently and were selected for further study. The accessibility of bound aflatoxin B(1) to an antibody in an indirect competitive inhibition enzyme-linked immunosorbent assay suggests that surface components of these bacteria are involved in binding. Further evidence is the recovery of around 90% of the bound aflatoxin from the bacteria by solvent extraction. Autoclaving and sonication did not release any detectable aflatoxin B(1). Variation in temperature (4 to 37 degrees C) and pH (2 to 10) did not have any significant effect on the amount of aflatoxin B(1) released. Binding of aflatoxin B(1) appears to be predominantly extracellular for viable and heat-treated bacteria. Acid treatment may permit intracellular binding. In all cases, binding is of a reversible nature, but the stability of the complexes formed depends on strain, treatment, and environmental conditions.
Authors:
C A Haskard; H S El-Nezami; P E Kankaanpää; S Salminen; J T Ahokas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  67     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-06-26     Completed Date:  2001-09-20     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3086-91     Citation Subset:  IM    
Affiliation:
Key Centre for Applied and Nutritional Toxicology, School of Medical Sciences, RMIT-University, Bundoora, Victoria 3083, Australia. carolyn.haskard@sawater.sa.gov.au
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MeSH Terms
Descriptor/Qualifier:
Aflatoxin B1 / metabolism*
Culture Media
Enzyme-Linked Immunosorbent Assay
Hot Temperature
Hydrogen-Ion Concentration
Lactobacillus / growth & development,  metabolism*
Lactococcus / classification,  growth & development,  metabolism*
Protein Binding
Chemical
Reg. No./Substance:
0/Culture Media; 1162-65-8/Aflatoxin B1
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