Document Detail


Surface lipoprotein PpiA of Streptococcus mutans suppresses scavenger receptor MARCO-dependent phagocytosis by macrophages.
MedLine Citation:
PMID:  21986627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptococcus mutans is associated with the initiation and progression of human dental caries and is occasionally isolated from the blood of patients with bacteremia and infective endocarditis. For the pathogen to survive in the infected host, surface lipoproteins of S. mutans are likely to play important roles in interactions with the innate immune system. To clarify the role that a putative lipoprotein, peptidyl-prolyl cis/trans-isomerase (PpiA), of S. mutans plays in the macrophage response, we investigated the response of THP-1-derived macrophages to S. mutans challenge. The deletion of the gene encoding Lgt eliminated PpiA on the cell surface of S. mutans, which implies that PpiA is a lipoprotein that is lipid anchored in the cell membrane by Lgt. Human and murine peritoneal macrophages both showed higher phagocytic activities for the ppiA and lgt mutants than the wild type, which indicates that the presence of PpiA reduces S. mutans phagocytosis. In addition, infection with S. mutans markedly induced mRNAs of macrophage receptor with collagenous structure (MARCO) and scavenger receptor A (SR-A) in human macrophages. In particular, transcriptional and translational levels of MARCO in human macrophages infected with the ppiA mutant were higher than those in macrophages infected with the wild type. Phagocytosis of S. mutans by human macrophages markedly decreased after treatment with anti-MARCO IgG. These results demonstrate that the S. mutans lipoprotein PpiA contributes to suppression of MARCO-mediated phagocytosis of this bacterium by macrophages.
Authors:
Tadashi Mukouhara; Takafumi Arimoto; Kasei Cho; Matsuo Yamamoto; Takeshi Igarashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-10-10
Journal Detail:
Title:  Infection and immunity     Volume:  79     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-14     Completed Date:  2012-01-13     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4933-40     Citation Subset:  IM    
Affiliation:
Department of Oral Microbiology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Bacterial Outer Membrane Proteins / genetics,  metabolism*
Cell Line, Tumor
Cyclophilin A / metabolism*
Gene Expression Regulation / physiology
Humans
Immunoglobulin G / immunology
Macrophages / physiology*
Mice
Mutation
Phagocytosis / drug effects*,  physiology
Receptors, Immunologic / genetics,  metabolism*
Scavenger Receptors, Class A / genetics,  metabolism
Streptococcus mutans / immunology,  metabolism*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Bacterial Outer Membrane Proteins; 0/Immunoglobulin G; 0/MARCO protein, human; 0/Receptors, Immunologic; 0/Scavenger Receptors, Class A; EC 5.2.1.-/Cyclophilin A
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