| Surface ligand effects on metal-affinity coordination to quantum dots: implications for nanoprobe self-assembly. | |
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MedLine Citation:
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PMID: 20568725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The conjugation of biomolecules such as proteins and peptides to semiconductor quantum dots (QD) is a critical step in the development of QD-based imaging probes and nanocarriers. Such protein-QD assemblies can have a wide range of biological applications including in vitro protein assays and live-cell fluorescence imaging. One conjugation scheme that has a number of advantages is the self-assembly of biomolecules on a QD surface via polyhistidine coordination. This approach has been demonstrated using QDs that have different coating types, resulting in different interactions between the biomolecule and QD surface. Here, we report the use of a fluorescence resonance energy transfer (FRET) assay to evaluate the self-assembly of fluorescent proteins on the surface of QDs with eight distinct coatings, including several used in commercial preparations. The results of this systematic comparison can provide a basis for rational design of self-assembled biomolecule-QD complexes for biomedical applications. |
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Authors:
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Allison M Dennis; David C Sotto; Bing C Mei; Igor L Medintz; Hedi Mattoussi; Gang Bao |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 21 ISSN: 1520-4812 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-29 Completed Date: 2010-11-16 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1160-70 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cadmium Compounds
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chemistry* Coated Materials, Biocompatible / chemical synthesis*, chemistry Fluorescence Resonance Energy Transfer Ligands Luminescent Proteins / chemistry Nanostructures / chemistry* Phospholipids / chemistry Polymers / chemistry Quantum Dots* Selenium Compounds / chemistry* Sulfides / chemistry* Surface Properties Zinc Compounds / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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1PN2EY018244/EY/NEI NIH HHS; CA119338/CA/NCI NIH HHS; HL80711/HL/NHLBI NIH HHS; PN2 EY018244-03/EY/NEI NIH HHS; PN2 EY018244-04/EY/NEI NIH HHS; U01 HL080711-04/HL/NHLBI NIH HHS; U01 HL080711-05/HL/NHLBI NIH HHS; U54 CA119338-040002/CA/NCI NIH HHS; U54 CA119338-050002/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cadmium Compounds; 0/Coated Materials, Biocompatible; 0/Ligands; 0/Luminescent Proteins; 0/Phospholipids; 0/Polymers; 0/Selenium Compounds; 0/Sulfides; 0/Zinc Compounds; 1306-24-7/cadmium selenide; 1314-98-3/zinc sulfide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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