| Supramolecularly Oriented Immobilization of Proteins Using Cucurbit[8]uril. | |
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MedLine Citation:
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PMID: 23134267 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A supramolecular strategy is used for oriented positioning of proteins on surfaces. A viologen based guest molecule is attached to the surface, while a naphthol guest moiety is chemoselectively ligated to a yellow fluorescent protein. CB[8] is used to link the proteins onto surfaces through specific charge-transfer interactions between naphthol and viologen inside the CB cavity. The assembly process is characterized using fluorescence and atomic force microscopy, surface plasmon resonance, IR-reflective absorption and X-ray photoelectron spectroscopy measurements. Two different immobilization routes are followed to form patterns of the protein ternary complexes on the surfaces. Each immobilization route consists of three steps; (i) attaching the viologen to the glass using microcontact chemistry, (ii) blocking and (iii) either incubation or microcontact printing of CB[8] and naphthol guests. In both cases uniform and stable fluorescent patterns are fabricated with a high signal to noise ratio. Control experiments confirm that CB[8] serves as a selective linking unit to form stable and homogeneous ternary surface-bound complexes as envisioned. The attachment of the YFP-complexes is shown to be reversible and reusable for assembly as studied using fluorescence microscopy. |
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Authors:
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Arantzazu Gonzalez-Campo; Melanie Brasch; Dana Uhlenheuer; Alberto Gomez-Casado; Lanti Yang; Luc Brunsveld; Jurriaan Huskens; Pascal Jonkheijm |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-7 |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: - ISSN: 1520-5827 ISO Abbreviation: Langmuir Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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