Document Detail


Supramolecularly Oriented Immobilization of Proteins Using Cucurbit[8]uril.
MedLine Citation:
PMID:  23134267     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A supramolecular strategy is used for oriented positioning of proteins on surfaces. A viologen based guest molecule is attached to the surface, while a naphthol guest moiety is chemoselectively ligated to a yellow fluorescent protein. CB[8] is used to link the proteins onto surfaces through specific charge-transfer interactions between naphthol and viologen inside the CB cavity. The assembly process is characterized using fluorescence and atomic force microscopy, surface plasmon resonance, IR-reflective absorption and X-ray photoelectron spectroscopy measurements. Two different immobilization routes are followed to form patterns of the protein ternary complexes on the surfaces. Each immobilization route consists of three steps; (i) attaching the viologen to the glass using microcontact chemistry, (ii) blocking and (iii) either incubation or microcontact printing of CB[8] and naphthol guests. In both cases uniform and stable fluorescent patterns are fabricated with a high signal to noise ratio. Control experiments confirm that CB[8] serves as a selective linking unit to form stable and homogeneous ternary surface-bound complexes as envisioned. The attachment of the YFP-complexes is shown to be reversible and reusable for assembly as studied using fluorescence microscopy.
Authors:
Arantzazu Gonzalez-Campo; Melanie Brasch; Dana Uhlenheuer; Alberto Gomez-Casado; Lanti Yang; Luc Brunsveld; Jurriaan Huskens; Pascal Jonkheijm
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-7
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  -     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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