| Supramolecular cocaine-aptamer complexes activate biocatalytic cascades. | |
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MedLine Citation:
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PMID: 19309141 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The anti-cocaine aptamer was fragmented into two nucleic acids, (1) and (2). The nucleic acid (1) was tethered at its 5'-end to aminoethyl nicotinamide adenine dinucleotide, amino-NAD(+), or to horseradish peroxidase, HRP. The nucleic acid (2) was functionalized at its 3'-end with alcohol dehydrogenase, AlcDH, or with glucose oxidase, GOx. In the presence of cocaine, the supramolecular NAD(+)/AlcDH/cocaine-aptamer complex is formed, and the biocatalytic oxidation of ethanol is activated. Similarly, in the presence of cocaine, the GOx/HRP/cocaine-aptamer complex is formed, and this activates the biocatalytic cascade where glucose is oxidized by GOx to yield gluconic acid and H(2)O(2), and the resulting hydrogen peroxide activates the HRP-biocatalyzed oxidation of 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate), ABTS(2-). The systems may be considered as biomimetic prototypes for systems biology. |
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Authors:
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Ronit Freeman; Etery Sharon; Ran Tel-Vered; Itamar Willner |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 131 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-08 Completed Date: 2009-06-24 Revised Date: 2009-07-09 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 5028-9 Citation Subset: IM |
Affiliation:
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Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Dehydrogenase
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chemistry,
metabolism* Aptamers, Nucleotide / chemistry, metabolism* Biocatalysis Biosensing Techniques / methods* Cocaine / analysis*, metabolism Ethanol / metabolism Gluconates / metabolism Glucose / metabolism Glucose Oxidase / chemistry, metabolism* Horseradish Peroxidase / chemistry, metabolism* Hydrogen Peroxide / metabolism NAD / chemistry, metabolism Oxidation-Reduction |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 0/Gluconates; 50-36-2/Cocaine; 50-99-7/Glucose; 526-95-4/gluconic acid; 53-84-9/NAD; 64-17-5/Ethanol; 7722-84-1/Hydrogen Peroxide; EC 1.1.1.1/Alcohol Dehydrogenase; EC 1.1.3.4/Glucose Oxidase; EC 1.11.1.-/Horseradish Peroxidase |
| Comments/Corrections | |
Erratum In:
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J Am Chem Soc. 2009 May 20;131(19):6886 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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