Document Detail

Suppressor mutants of Neurospora crassa that tolerate allelic differences at single or at multiple heterokaryon incompatibility loci.
MedLine Citation:
PMID:  8088519     Owner:  NLM     Status:  MEDLINE    
Allelic differences at any one of at least 11 heterokaryon incompatibility (het) loci in Neurospora crassa trigger an incompatibility response: localized cell death at sites of hyphal anastomosis. We have isolated spontaneous and insertional suppressor mutants that are heterokaryon-compatible in spite of allelic differences at one or at several het loci. Some intra- and extragenic mutants tolerated allelic differences only at single het loci. Multi-tolerant spontaneous mutants were isolated by selecting simultaneously for tolerance of differences at het-c, -d and -e, or at each of these plus mating-type. Some suppressor mutants were specific for only one allele at the affected het locus; others suppressed both alleles. Insertional mutations were isolated from banks of transformants, each having a plasmid integrated into a random position in the chromosome. One mutant tolerated allelic differences at het-d. A homologous cosmid from a Neurospora genomic bank complemented the mutant phenotype. A second insertional inactivation mutant was tolerant of het-c differences. Inactivation of the wild-type locus corresponding to the integration site was accomplished by repeat-induced point mutation (RIP). The RIP progeny, like the original mutant, were tolerant of differences at het-c. It may be possible to use such suppressor mutants as universal donors of hypovirulence in pathogenic fungi.
M T Arganoza; J Ohrnberger; J Min; R A Akins
Related Documents :
10559489 - Zic1 regulates the patterning of vertebral arches in cooperation with gli3.
10794599 - Isolation and characterization of a mutant defective in the production of methanol dehy...
9733529 - Wrinkled1: a novel, low-seed-oil mutant of arabidopsis with a deficiency in the seed-sp...
6596479 - The immi region of salmonella phage p22. 2. the influence of the cir mutations on the r...
3035339 - Cloning of seven differently complementing dna fragments with chl functions from escher...
12011349 - Mutants at the slender1 locus of barley cv himalaya. molecular and physiological charac...
3382759 - Cytogenetic investigations on children with acute non-lymphocytic leukemia.
2981609 - Localization of the oncogene c-ha-ras1 outside the aniridia-wilms' tumor-associated del...
8499899 - Human olfactory marker protein maps close to tyrosinase and is a candidate gene for ush...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  137     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-10-20     Completed Date:  1994-10-20     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  731-42     Citation Subset:  IM    
Wayne State University School of Medicine, Department of Biochemistry, Detroit, Michigan 48201.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Fusion
Chromosomes, Fungal
Crosses, Genetic
Genes, Fungal*
Genes, Mating Type, Fungal*
Genes, Suppressor*
Genetic Complementation Test
Mutagenesis, Insertional
Neurospora crassa / genetics*,  physiology
Point Mutation
Reproduction, Asexual
Spores, Fungal / physiology
Suppression, Genetic*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Superoxide dismutase (sod-1) null mutants of Neurospora crassa: oxidative stress sensitivity, sponta...
Next Document:  Structure, expression and phylogenetic analysis of the gene encoding actin I in Pneumocystis carinii...