Document Detail

Suppressor cells and intrathymic inoculation of donor alloantigens in cardiac transplantation.
MedLine Citation:
PMID:  8787463     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Donor-specific tolerance to a rat heterotopic cardiac allograft has been achieved by the pretransplantation intrathymic injection of donor splenocytes and a single intraperitoneal injection of antilymphocyte serum (ALS). Permanent tolerance is achieved without subsequent immunosuppression therapy. This study investigated the mechanisms responsible for maintenance of the tolerant state. METHODS: Tolerance was produced in Lewis rats by the administration of 1 mL of ALS intraperitoneally and 5 x 10(7) Lewis Brown Norway (LBN) splenocytes intrathymically 21 days before heterotopic transplantation using an LBN donor. RESULTS: In tolerant Lewis rats bearing LBN allografts for more than 100 days, rejection could not be produced by the intravenous injection of naive Lewis spleen cells (5 x 10(7) cells x 1 day, n = 5; 5 x 10(7) cells x 3 days, n = 5) or cells from Lewis rats sensitized to LBN tissues (5 x 10(7) cells x 3 days, n = 5). Naive Lewis recipients were pretreated with ALS and 6 days later with intravenous spleen cells (25 x 10(7), n = 5) or lymphoid cells (10 to 15 x 10(7), n = 5) from a tolerant animal bearing a viable LBN graft. When transplantation with an LBN donor was done the next day, significant prolongation of LBN allograft survival (mean survival time 32.8 days, p < 0.01; and 22.2 days, p < 0.01; respectively) was seen. Wistar-Furth allograft survival was not prolonged by treatment with ALS and intravenous spleen (n = 5) or lymph node (n = 5) cells from rats tolerant to LBN tissues (mean survival time 8.6 and 9.2 days, control 9 days; p = not significant). The administration of ALS alone (n = 5) did not prolong LBN graft survival (mean survival time 11.8 days). CONCLUSION: These data suggest that transferable suppressor cells specific for the donor strain are at least in part responsible for the maintenance of long-term allograft survival after intrathymic pretreatment with allogeneic cells.
Z Shen; M Mohiuddin; V J DiSesa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  60     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-09-24     Completed Date:  1996-09-24     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1683-5     Citation Subset:  AIM; IM    
Department of Surgery, Medical College of Pennsylvania, Philadelphia 19129, USA.
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MeSH Terms
Antilymphocyte Serum / administration & dosage,  immunology
Cell Transplantation
Graft Rejection
Heart Transplantation / immunology*
Immune Tolerance
Immunization, Passive
Isoantigens / administration & dosage*,  immunology
Rats, Inbred BN
Rats, Inbred Lew
Rats, Inbred WF
Spleen / cytology
T-Lymphocytes, Regulatory / immunology*
Thymus Gland
Tissue Donors
Transplantation, Heterotopic
Transplantation, Homologous
Reg. No./Substance:
0/Antilymphocyte Serum; 0/Isoantigens

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