Document Detail


Suppressive effects of swainsonine on C6 glioma cell in vitro and in vivo.
MedLine Citation:
PMID:  19427771     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Swainsonine, an extract from Astragalus membranaceus, is known for its anti-cancer effects and could prevent metastases. In order to investigate the effects and mechanisms of swainsonine in C6 glioma cells, we carry out correlated experiments in vitro and in vivo. After treatment with swainsonine, the effective dose and IC(50) value of swainsonine in the C6 glioma cell were examined using the MTT assay. Cell cycle distribution and apoptotic rates were analyzed using FCM and [Ca(2+)](i) was measured by LSCM. Expressions of p16 and p53 protein were evaluated by immunocytochemical methods. Simultaneously, glioma-bearing rats were administered swainsonine at doses of 2, 4 and 8 mg/kg body wt. The inhibition rate was calculated and pathological sections were observed. The results indicated that the growth of C6 glioma cells is inhibited by swainsonine in vitro, with an IC(50) value within 24h of 0.05 microg/ml. Increases in swainsonine correlate with S phase percentages of 11.3%, 11.6% and 12.4%, respectively. Moreover, the expression of apoptosis inhibiting p53 and p16 protein decreases gradually. Tumor weight in vivo decreased clearly and HE dyeing of tumor tissue showed gray, its texture was soft, with necrosis and hemorrhagic concentrated inward. Swainsonine could inhibit the proliferation of C6 glioma cells in vitro and the growth of C6 glioma in vivo. The mechanisms of swainsonine-induced apoptosis may relate with the expression of apoptosis-related genes and overloading-[Ca(2+)](i)-induced endoplasmic reticulum stress.
Authors:
Ji-Yuan Sun; Hao Yang; Shan Miao; Ji-Peng Li; Si-Wang Wang; Miao-Zhang Zhu; Yan-Hua Xie; Jian-Bo Wang; Zhe Liu; Qian Yang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-08
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  16     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-12     Completed Date:  2010-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1070-4     Citation Subset:  IM    
Affiliation:
Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Phytogenic / pharmacology*,  therapeutic use
Apoptosis / drug effects*,  genetics
Astragalus Plant
Calcium / metabolism
Cell Cycle / drug effects
Cell Line, Tumor
Genes, p16 / drug effects
Genes, p53 / drug effects
Glioma / drug therapy*,  metabolism
Male
Phytotherapy*
Plant Extracts / pharmacology,  therapeutic use
Rats
Rats, Sprague-Dawley
Swainsonine / pharmacology*,  therapeutic use
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Plant Extracts; 72741-87-8/Swainsonine; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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