Document Detail

Suppressive effects of Okinawan food items on free radical generation from stimulated leukocytes and identification of some active constituents: implications for the prevention of inflammation-associated carcinogenesis.
MedLine Citation:
PMID:  16435988     Owner:  NLM     Status:  MEDLINE    
Okinawa prefecture in Japan is a distinct area characterized by unique traditional food habits and longevity. Prolonged exposure to activated leukocytes, playing pivotal roles in chronic inflammation-associated carcinogenesis, is known to lead to oxidative and nitrosative damage to macromolecules in the body since they are primary sources of free radicals, such as superoxide anion (O(2)(-)) and nitric oxide (NO). In this study, we estimated anti-oxidative and anti-nitrosative activities of Okinawan food items by employing two cellular experimental systems: (1) phorbol ester-induced O(2)(-) generation from differentiated HL-60 human promyelocytic leukemia cells; and (2) lipopolysaccharide (LPS)-induced NO generation in RAW264.7 murine macrophages. A total of 138 food items, consisting of 42 samples unique to Okinawa and 96 common in the Japanese main island, were purchased at local markets in Okinawa and extracted with chloroform. When tested at a concentration of 100 microg/ml, 38% (16/42) of the former showed 70% or more inhibition of O(2)(-) generation while 21% (20/96) of the latter did so. In parallel, 64% (27/42) of the former showed significant NO generation suppression in contrast to 48% (46/96) of the latter . Twenty-one active species were further tested at a concentration of 20 mug/ml, and eleven species, including sugar cane, wild turmeric, and zedoary, were indicated to be most promising items with anti-oxidative and anti-nitrosative properties. In addition, some of the active constituents (chebulagic acid, a resveratrol derivative, and sesquiterpenoids) were identified. Our results suggest that food items typical in the Okinawa area have higher cancer preventive potential than those common in Japan.
Akira Murakami; Hisashi Ishida; Kimie Kobo; Ikuyo Furukawa; Yasutaka Ikeda; Megumi Yonaha; Yohko Aniya; Hajime Ohigashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Asian Pacific journal of cancer prevention : APJCP     Volume:  6     ISSN:  1513-7368     ISO Abbreviation:  Asian Pac. J. Cancer Prev.     Publication Date:    2005 Oct-Dec
Date Detail:
Created Date:  2006-01-26     Completed Date:  2006-05-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101130625     Medline TA:  Asian Pac J Cancer Prev     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  437-48     Citation Subset:  IM    
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
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MeSH Terms
Cell Culture Techniques
Granulocyte Precursor Cells / drug effects*,  metabolism
HL-60 Cells
Macrophages / drug effects*,  metabolism
Nitric Oxide / metabolism*
Plant Extracts / pharmacology
Plant Structures
Plants, Edible*
Sesquiterpenes / pharmacology
Stilbenes / pharmacology
Superoxides / metabolism*
Reg. No./Substance:
0/Plant Extracts; 0/Sesquiterpenes; 0/Stilbenes; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides

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