Document Detail


Effect of suppressive oligodeoxynucleotides on the development of inflammation-induced papillomas.
MedLine Citation:
PMID:  21367957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inflammation contributes to the development of papillomas and squamous cell carcinomas in the well-established 7,12-dimethylbenz(a)anthracene (DMBA)/12-O-tetradecanoylphorbol-l3-acetate (TPA) model of skin carcinogenesis. Synthetic oligonucleotides (ODN) containing repetitive TTAGGG motifs have been shown to block deleterious inflammatory reactions in murine models of autoimmunity, pneumonitis, and shock. This article examines whether treatment with suppressive (Sup) ODN can interfere with DMBA/TPA-induced inflammation, thereby reducing papilloma formation. Results indicate that Sup ODN block TPA-dependent skin hyperplasia, edema, and leukocytic infiltration. Sup ODN also inhibit the upregulation of genes encoding pro-oncogenic chemokines and other markers of inflammation including CXCL2, CCL2, COX-2, and ODC (ornithine decarboxylase). Of greatest import, Sup ODN reduce papilloma formation in a dose- and sequence-dependent manner. These findings suggest that Sup ODN may provide a novel means of preventing inflammation and associated oncogenesis.
Authors:
Hidekazu Ikeuchi; Takeshi Kinjo; Dennis M Klinman
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Publication Detail:
Type:  Journal Article     Date:  2011-03-02
Journal Detail:
Title:  Cancer prevention research (Philadelphia, Pa.)     Volume:  4     ISSN:  1940-6215     ISO Abbreviation:  Cancer Prev Res (Phila)     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-05     Completed Date:  2011-09-27     Revised Date:  2012-05-02    
Medline Journal Info:
Nlm Unique ID:  101479409     Medline TA:  Cancer Prev Res (Phila)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  752-7     Citation Subset:  IM    
Affiliation:
Cancer and Inflammation Program, National Cancer Institute, Frederick, MD 21702, USA.
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MeSH Terms
Descriptor/Qualifier:
9,10-Dimethyl-1,2-benzanthracene / toxicity
Animals
Chemokine CCL2 / genetics,  metabolism
Chemokine CXCL2 / genetics,  metabolism
Cyclooxygenase 2 / genetics,  metabolism
Female
Inflammation / chemically induced,  genetics,  prevention & control*
Mice
Oligodeoxyribonucleotides / therapeutic use*
Ornithine Decarboxylase / genetics,  metabolism
Papilloma / chemically induced,  genetics,  prevention & control*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Skin Neoplasms / chemically induced,  genetics,  prevention & control*
Tetradecanoylphorbol Acetate / toxicity
Grant Support
ID/Acronym/Agency:
ZIA BC010853-04/BC/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Chemokine CCL2; 0/Chemokine CXCL2; 0/Oligodeoxyribonucleotides; 0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate; 57-97-6/9,10-Dimethyl-1,2-benzanthracene; EC 1.14.99.-/Ptgs2 protein, mouse; EC 1.14.99.1/Cyclooxygenase 2; EC 4.1.1.17/Ornithine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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