Document Detail

Suppression of tif-mediated induction of SOS functions in Escherichia coli by an altered dnaB protein.
MedLine Citation:
PMID:  159289     Owner:  NLM     Status:  MEDLINE    
The tif-1 mutation in the Escherichia coli recA gene is known to cause induction of the various "SOS" functions at high temperature, including massive synthesis of the recA protein, lethal filamentation, elevated mutagenesis, and, in lambda lysogens, induction of prophage. It is shown here that the deoxyribonucleic acid initiation mutation dnaB252 suppresses all these manifestations of tif expression. Induction of lambda by ultraviolet irradiation, however, is not affected by the dnaB252 mutation. No similar suppression of tif is observed with other dnaB mutations affecting deoxyribonucleic acid elongation or with other deoxyribonucleic acid initiation mutations at the dnaA and dnaC loci. The fact that an alteration of the dnaB protein specifically suppresses tif-mediated SOS induction implies a role of the replication apparatus in this process, as has been suggested for ultraviolet induction. The induction of lambda is known to proceed via repressor cleavage, presumably promoted by an activated (protease) form of the recA protein. Since lambda induction is normal after ultraviolet irradiation of the tif-1 dnaB252(lambda) strain, tif-mediated induction in this strain may be blocked in a tif-specific step leading to activation of the recA (tif) protein. It is possible that the recA (tif) mutant protein may be directly involved in the replication complex in processes leading to this activation.
R D'Ari; J George; O Huisman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  140     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1979 Nov 
Date Detail:
Created Date:  1980-01-28     Completed Date:  1980-01-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
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MeSH Terms
Bacterial Proteins / genetics,  physiology*
Bacteriophage lambda / growth & development
DNA Replication*
Escherichia coli / genetics,  physiology*
Reg. No./Substance:
0/Bacterial Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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