Document Detail


Suppression of telomerase activity by pyrimethamine: implication to cancer.
MedLine Citation:
PMID:  18392083     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Although pyrimethamine (Tindurin) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. We design this study to evaluate its anti-tumoral effect on a model of cell line. METHODS: The cytotoxic influence of Pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. The potential modulatory effects on metastasis, apoptosis, and immortality characteristics of cells were assessed with gelatin zymography, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and telomeric repeat amplification protocol, respectively. RESULTS: Cytotoxicity analysis of pyrimethamine revealed a dose-dependent fashion. An apoptotic influence of pyrimethamine was also confirmed by data obtained from TUNEL assay. Dose-dependent inhibitory effect on matrix metalloproteinases (MMP) was seen in pyrimethamine. A potent inhibitory effect of pyrimethamine was also established by data achieved from TRAPeze telomerase detection kit. CONCLUSIONS: Collectively, as induction of apoptosis together with MMP and telomerase inhibition could be indicative of cancer treatment, pyrimethamine might be considered as a chemopreventative agent in cancer.
Authors:
Mohammad Reza Khorramizadeh; Farshid Saadat; Farhad Vaezzadeh; Farnaz Safavifar; Hassan Bashiri; Zahra Jahanshiri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Iranian biomedical journal     Volume:  11     ISSN:  1028-852X     ISO Abbreviation:  Iran. Biomed. J.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2008-04-08     Completed Date:  2008-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9814853     Medline TA:  Iran Biomed J     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  223-8     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, School of Public Health, Medical Sciences/University of Tehran, P.O. Box: 14155-6446, Tehran, Iran. khoramza@sina.tums.ac.ir
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Cell Line, Tumor
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology*
Humans
Male
Matrix Metalloproteinase 2 / metabolism
Pyrimethamine / pharmacology*
Telomerase / antagonists & inhibitors*
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 58-14-0/Pyrimethamine; EC 2.7.7.49/Telomerase; EC 3.4.24.24/Matrix Metalloproteinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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