Document Detail

Suppression of telomerase activity and arrest at G1 phase in human cervical cancer HeLa cells by all-trans retinoic acid.
MedLine Citation:
PMID:  16445656     Owner:  NLM     Status:  MEDLINE    
Of all neoplasms found in women, cervical cancer has the third highest incidence and causes the fourth most deaths. All-trans retinoic acid (ATRA) may be with chemopreventive potential on cervical cancer, but the mechanisms underlying is not clear. To investigate the mechanisms, human cervical cancer HeLa cells were treated with ATRA for 1, 2, 3, or 4 days in vitro. We found that ATRA inhibited the growth of HeLa cells in a dose-dependent manner at the concentrations from 0.3 to 9.6 mumol/L. Flow cytometric analysis showed that HeLa cells were arrested at G0/G1 phase by ATRA, and the aneuploidy was found when cells were treated for 4 days, which is the first report that ATRA causes aneuploid cycle in HeLa cells. The expression of human telomerase catalytic subunit messenger RNA was decreased remarkably by ATRA. These findings suggested that the inhibition of telomerase activity and arrest of cells at G0/G1 phase might be the key steps through which ATRA inhibits the proliferation of HeLa cells. Our results provide a possible mechanistic explanation for the growth inhibitory effect of ATRA on HeLa cells. Therefore, retinoids may have therapeutic potential to complement current treatments of cervical cancers.
J M Guo; B X Xiao; G Z Kang; D H Liu; H Chen; S Zhang; X N Zhang
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of gynecological cancer : official journal of the International Gynecological Cancer Society     Volume:  16     ISSN:  1048-891X     ISO Abbreviation:  Int. J. Gynecol. Cancer     Publication Date:    2006 Jan-Feb
Date Detail:
Created Date:  2006-01-31     Completed Date:  2006-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9111626     Medline TA:  Int J Gynecol Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  341-6     Citation Subset:  IM    
School of Medicine, Ningbo University, Ningbo 315211, China.
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MeSH Terms
Apoptosis / drug effects
Cell Cycle / drug effects*
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
G1 Phase / drug effects*,  physiology
Hela Cells / drug effects
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Telomerase / drug effects,  metabolism*
Tretinoin / pharmacology*
Tumor Cells, Cultured
Tumor Markers, Biological / analysis
Uterine Cervical Neoplasms / drug therapy,  pathology
Reg. No./Substance:
0/RNA, Messenger; 0/Tumor Markers, Biological; 302-79-4/Tretinoin; EC

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